Scanning Microscope, and Method for Light Microscopy Imaging of a Specimen
First Claim
1. A scanning microscope havingan illumination unit for emitting an illumination light beam,an objective for generating an elongated illumination focus in a specimen to be imaged, anda scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective,wherein the scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis (O) of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated.
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Abstract
A scanning microscope is described, having an illumination unit for emitting an illumination light beam, an objective for generating an elongated illumination focus in a specimen to be imaged, and a scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective. The scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated.
32 Citations
20 Claims
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1. A scanning microscope having
an illumination unit for emitting an illumination light beam, an objective for generating an elongated illumination focus in a specimen to be imaged, and a scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective, wherein the scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis (O) of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated.
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15. A method for light microscopy imaging of a specimen, having the following steps:
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emitting an illumination light beam, generating an elongated illumination focus in the specimen to be imaged by means of an objective, moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective, wherein the illumination light beam is directed onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis (O) of the objective, and the direction of incidence of the illumination light beam is modified within that sub-region in order to move the illumination focus over the target region to be illuminated. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification