SYNBIOTIC COMPOSITIONS FOR RESTORATION AND RECONSTITUTION OF GUT MICROBIOTA

• US 20130336931A1
• Filed: 02/09/2012
• Published: 12/19/2013
• Est. Priority Date: 02/09/2011
• Status: Active Grant

• Current Assignee: Synbiotics AB
• Original Assignee: Lavivo AB (Aptestart AB)
• Inventors: Wadstrm, Torkel, Ljungh, Asa, Ambalam, Padma, Kondepudi, Kanthi Kiran
• Granted Patent: US 8,927,252 B2
• Time in Patent Office: Days
• US Class Current: 424/93.3
• CPC Class Codes: A23L 29/30 containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin products from apiculture A23L21/20; artificial sweetening agents A23L27/30
• CPC Class Codes: A23L 33/125 containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates indigestible substances A23L33/21
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A23L 33/21 Addition of substantially indigestible substances, e.g. dietary fibres
• CPC Class Codes: A23L 5/00 Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2200/3202 Prebiotics, ingredients fermented in the gastrointestinal tract by beneficial microflora
• CPC Class Codes: A23V 2200/3204 Probiotics, living bacteria to be ingested for action in the digestive tract
• CPC Class Codes: A23V 2250/28 Oligosaccharides
• CPC Class Codes: A23V 2250/612 Lactose
• CPC Class Codes: A23V 2400/165 Paracasei
• CPC Class Codes: A23V 2400/519 Breve
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 36/81 Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/12 Antidiarrhoeals
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates to synbiotic compositions comprising probiotic bacterial strains and prebiotic substances that, when combined exhibit synergistic behavior. The synergetic compositions will stimulate the indigenous microflora to restore and reconstitute in vivo gut like conditions after antibiotic associated diarrhea (AAD), and/or other gut infections caused by gastrointestinal pathogens, and relapses thereof, as well as the prevention of said disorders.

Claims

1-33. -33. (canceled)

34. A synbiotic composition comprising at least two bacterial strains selected from the group consisting of the Lactobacillus and Bifidobacteria genus, and one or more prebiotic substances, wherein:

at least one of the bacterial strains is Bifidobacterium breve Bif LU 10 (deposit no;

LMG P-26117) and capable of degrading starch; and

at least one prebiotic substance is starch.

35. The synbiotic composition according to claim 34, wherein the starch is resistant starch (R8) and/or soluble starch (88).

36. The synbiotic composition according to claim 34, wherein one of said bacterial strains is a non-starch degrading strain.

37. The synbiotic composition according to claim 36, wherein the non-starch degrading bacterial strain is of the Lactobacillus paracasei species.

38. The synbiotic composition according to claim 36, wherein the non-starch degrading bacterial strain is LAB LU 33 (deposit no:

LMG P-26118).

39. The synbiotic composition according to claim 34, further comprising one or more of the strains LAB LU 23 (deposit no:

LMG-P-26119), LAB LU 28 (deposit no;

LMG P-26120), BIF LU 29 (deposit no;

LMG P-26115), and/or BIF LU 30 (deposit no;

LMG P-26116).

40. The synbiotic composition according to claim 39, comprising at least LAB LU 33 (deposit no:

LMG P-26118), and BIF LU 30 (deposit no;

LMG P-26116).

41. The synbiotic composition according to claim 39, comprising at least LAB LU 33 (deposit no:

LMG P-26118), BIF LU 30 (deposit no;

LMG P-26116), and LAB LU 28 (deposit no;

LMG P-26120).

42. The synbiotic composition according to claim 34, wherein one or more of the bacterial strains has been exposed to acid, bile and/or mucin during their production.

43. The synbiotic composition according to claim 34, further comprising a prebiotic substance of the group consisting of disaccharides, oligosaccharides, and/or polysaccharides.

44. A synbiotic composition according to claim 34, for use in the colonization of the intestinal mucosa of a subject.

45. A synbiotic composition according to claim 34, for use in the prevention, treatment of, amelioration of symptoms, and/or prevention of relapse of antibiotic-associated diarrhea (AAD) and/or infections caused by gastrointestinal pathogens in a subject.

46. The synbiotic composition for use according to claim 45, wherein the antibiotic-associated diarrhea (AAD) is induced by Clostridium difficile.

47. The synbiotic composition for use according to claim 45, wherein the infection by gastrointestinal pathogens is caused by Salmonella, Campylobacter jejuni, Extended Spectrum Beta Lactamase producing (ESBL) E. coli.

48. The synbiotic composition for use according to claim 44, wherein the subject is a mammal.

49. A method for the colonization of the intestinal mucosa of a subject, said method comprising the step of administering the synbiotic composition of claim 34 in an effective dose to a subject in need thereof.

50. A method for treating a subject for antibiotic-associated diarrhea (AAD) and/or infections caused by gastrointestinal pathogens, said method comprising the step of administering the synbiotic composition of claim 34 in an effective dose to a subject in need thereof.