FLEXIBLE BAG FOR CULTIVATION OF CELLS
First Claim
Patent Images
1. An inflatable bioreactor bag (1) for cell cultivation comprising a top (2;
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22) and a bottom (3;
23) sheet of flexible material, joined together to form two end edges (4) and two side edges (5), wherein one baffle (6) or a plurality of baffles (6) extend from said bottom sheet in a region where the shortest distance to any one of said two end edges (4) is higher than about one fourth of the shortest distance between said two end edges.
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Abstract
An inflatable bioreactor bag for cell cultivation, which comprising a top and a bottom sheet of flexible material, joined together to form two end edges and two side edges, wherein one baffle or a plurality of baffles extend from the bottom sheet in a region where the shortest distance to any one of the two end edges is higher than about one fourth of the shortest distance between the two end edges.
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Citations
23 Claims
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1. An inflatable bioreactor bag (1) for cell cultivation comprising a top (2;
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22) and a bottom (3;
23) sheet of flexible material, joined together to form two end edges (4) and two side edges (5), wherein one baffle (6) or a plurality of baffles (6) extend from said bottom sheet in a region where the shortest distance to any one of said two end edges (4) is higher than about one fourth of the shortest distance between said two end edges. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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22) and a bottom (3;
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14. A method for cell cultivation comprising the steps of:
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a) providing an inflatable bioreactor bag comprised of a top and a bottom sheet of polymer material, joined together to form two end edges (4) and two side edges (5), wherein one baffle (6) or a plurality of baffles (6) extend from said bottom sheet in a region where the shortest distance to any one of said two end edges is higher than about one fourth of the distance between said two end edges; b) mounting said bioreactor bag on a support pivotally mounted to a base about a movable axis generally parallel to said end edges; c) providing cell culture media and a cell inoculate to said bag; and d) cultivating cells in the bag with agitation provided by rocking said support around said movable axis, forming a cell suspension and supplying at least one gas via a port in said top sheet. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification