Bioactive Peptides and Proteins Containing Bioactive Peptides, their Uses and Processes for Making the Same

• US 20140011742A1
• Filed: 12/08/2011
• Published: 01/09/2014
• Est. Priority Date: 12/09/2010
• Status: Abandoned Application

• Current Assignee: McGill University
• Original Assignee: McGill University
• Inventors: Sedman, Jacqueline, Ismail, Ashraf A., Gomaa, Ahmed I.
• Application Number:
  US13/992,685
• Publication Number:
  US 20140011742A1
• Time in Patent Office: Days
• US Class Current: 514/15.7
• CPC Class Codes: A23J 3/08 Dairy proteins
• CPC Class Codes: A23J 3/16 from soybean
• CPC Class Codes: A23L 33/18 Peptides; Protein hydrolysates
• CPC Class Codes: A23L 33/19 Dairy proteins
• CPC Class Codes: A23L 5/34 using microwaves
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2200/322 having an effect on the health of the nervous system or on mental function
• CPC Class Codes: A23V 2200/326 having effect on cardiovascular health
• CPC Class Codes: A23V 2250/54242 Alpha lactalbumin
• CPC Class Codes: A23V 2250/54244 Beta lactoglobulin
• CPC Class Codes: A23V 2250/5488 Soybean protein
• CPC Class Codes: A61K 38/556 Angiotensin converting enzyme inhibitors
• CPC Class Codes: A61P 9/12 Antihypertensives
• CPC Class Codes: C07K 1/12 by hydrolysis , i.e. solvolysis in general
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/4717 Plasma globulins, lactoglobulin
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00
• CPC Class Codes: C12Y 304/00 Hydrolases acting on peptide bonds, i.e. peptidases (3.4)

Abstract

A process for treating a protein before hydrolytic digestion, the process comprising exposing the protein to at least one cycle of microwave irradiation to produce a microwave treated protein containing one or more bioactive peptides. Further hydrolyzing the microwave treated protein to release at least one of the one or more bioactive peptides. A pharmaceutical composition, supplement and food product including the microwave treated protein or the one or more released bioactive peptides.

Claims

1. A process for treating a protein before hydrolytic digestion, the process comprising exposing the protein to at least one cycle of microwave irradiation to produce a microwave treated protein containing one or more bioactive peptides.

2. The process of claim 1, further comprising hydrolytic digestion of the microwave treated protein with at least one proteolytic enzyme to release at least one of the one or more bioactive peptides.

3. The process of claim 2, further comprising terminating said hydrolytic digestion in order to release the one or more bioactive peptides.

4. The process of claim 1, wherein the protein is a food protein or a protein derived from food ingredient.

5. The process of claim 4, wherein the food protein is a whey protein or a soy protein.

6. The process of claim 5, wherein the whey protein is β

-lactoglobulin or α

-lactalbumin.

8. The process of claim 1, wherein the bioactive peptide is VLDTDYKYLL (SEQ ID NO:

1) or TPPVDDEALEK (SEQ ID NO;

2).

10. The process of claim 1, wherein the microwave irradiation is carried out at a frequency of between about 0.3 to about 300 GHz, preferably 2.45 GHz.

11. The process of claim 1, wherein the microwave irradiation is carried out at a power of between about 1 and 6000 W, preferably between 20 and 6000 W, and most preferably at 30 W.

12. The process of claim 1, wherein the protein is subjected to a temperature of at least 30°

C., preferably between about 30 to 90°

C., during the microwave irradiation.

13. The process of claim 1, wherein the protein is exposed to the microwave irradiation for from about 1 to about 100 cycles, preferably for about 1 to about 10 cycles, of about 0.1 to about 20 seconds.

15. The process of claim 2, wherein the proteolytic enzyme is a pepsin, trypsin, chymotrypsin or mixtures thereof.

16. The process of claim 12, wherein said hydrolytic digestion is carried out sequentially with at least two different proteolytic enzymes.

17. The process of claim 13, wherein the proteolytic enzyme is used at a ratio from 1:

20 to 1;

250.

18. The process of claim 1, wherein the concentration of the protein is in the range of about 1 to about 35%, preferably about 1 to about 10%, most preferably about 5%.

32. A method for modulating hypertension in a mammal comprising administering to said mammal the microwave treated protein of claim 1.

34. A method for inhibiting ACE activity in a mammal comprising administering to said mammal the microwave treated protein of claim 1.

7. (canceled)

9. (canceled)

14. (canceled)

19-31. -31. (canceled)

33. (canceled)

35-42. -42. (canceled)