METHODS FOR GENETIC CONTROL OF INSECT INFESTATIONS IN PLANTS AND COMPOSITIONS THEREOF
First Claim
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1. A recombinant polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824;
(b) a polynucleotide that hybridizes to a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824 under wash conditions of 5×
SSC, 50% formamide and 42°
C. for 10 minutes;
(c) a polynucleotide comprising at least 70% sequence identity to a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824;
(d) a fragment of at least 21 contiguous nucleotides of a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824, wherein ingestion by a coleopteran plant pest of a double stranded ribonucleotide sequence comprising at least one strand that is complementary to said fragment inhibits the growth of said pest; and
(e) a complement of the sequence of (a), (b), (c) or (d).
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Abstract
The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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Citations
38 Claims
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1. A recombinant polynucleotide selected from the group consisting of:
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(a) a polynucleotide comprising a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824;(b) a polynucleotide that hybridizes to a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824 under wash conditions of 5×
SSC, 50% formamide and 42°
C. for 10 minutes;(c) a polynucleotide comprising at least 70% sequence identity to a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824;(d) a fragment of at least 21 contiguous nucleotides of a nucleic acid sequence of SEQ ID NO;
818, 820, 822, 823 or 824, wherein ingestion by a coleopteran plant pest of a double stranded ribonucleotide sequence comprising at least one strand that is complementary to said fragment inhibits the growth of said pest; and(e) a complement of the sequence of (a), (b), (c) or (d). - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 35, 36, 37, 38)
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2. (canceled)
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14. (canceled)
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19-34. -34. (canceled)
Specification