BACTERIAL CULTURE MEDIA AND METHODS FOR THEIR PREPARATION AND USE

• US 20140024075A1
• Filed: 05/15/2012
• Published: 01/23/2014
• Est. Priority Date: 05/15/2012
• Status: Active Grant

• Current Assignee: Shanghai Zhiyi Information Technology Limited
• Original Assignee: Shanghai Zhiyi Information Technology Limited
• Inventors: Hong, Feng
• Granted Patent: US 9,708,580 B2
• Time in Patent Office: Days
• US Class Current: 435/68.1
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase

Abstract

Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller'"'"'s grain.

Claims

1. A method of making culture medium for bacteria, the method comprising:

providing distiller'"'"'s grain from an alcohol fermentation; and

mixing the distiller'"'"'s grain with at least one hydrolytic catalyst, wherein a hydrolysate is formed from the distiller'"'"'s grain by the hydrolytic catalyst, thereby making a culture medium.

2. The method of claim 1, further comprising collecting the hydrolysate from the mixture.

3. The method of claim 2, further comprising detoxifying the hydrolysate.

4. The method of claim 1, further comprising providing at least one of a carbon source, a nitrogen source, a salt source, and a trace element source.

5. The method of claim 1, wherein the hydrolytic catalyst comprises an acid or an enzyme.

6. The method of claim 1, wherein a ratio of solids to liquids in the mixing of the distiller'"'"'s grain with the at least one hydrolytic catalyst is about 1:

5 (w/v) to about 1;

30 (w/v).

7. The method of claim 6, wherein the distiller'"'"'s grain is mixed with about 0.3% to about 0.7% w/v acid.

8. The method of claim 7, wherein the acid is mixed with the distiller'"'"'s grain at about 25 to about 200 degrees Celsius for about 30 to about 80 minutes.

10. The method of claim 6, wherein the distiller'"'"'s grain is mixed with about 1 to about 700 U of at least one enzyme.

11. The method of claim 10, wherein the enzyme is mixed with the distiller'"'"'s grain at about 25 to about 90 degrees Celsius for about 0.5 to about 48 hours.

12. The method of claim 11, wherein the enzyme is at least one of cellulase, hemicellulase, xylanase, protease, lipase, amylase, glucan glucohydrolase, or glucoamylase.

15. The method of claim 1, wherein the distiller'"'"'s grain is at least one of wet distiller'"'"'s grain (WDG), distiller'"'"'s dried grains (DDG), distiller'"'"'s dried solubles (DDS), or distiller'"'"'s dried grains with solubles (DDGS).

16. The method of claim 1, wherein the distiller'"'"'s grain includes residual ethanol.

9. (canceled)

13. (canceled)

14. (canceled)

17. (canceled)

18. A method of producing bacterial cellulose, the method comprising:

inoculating at least one bacterium in a culture medium, wherein the culture medium comprises a distiller'"'"'s grain;

cultivating the at least one bacterium in the culture medium to produce bacterial cellulose; and

harvesting the bacterial cellulose.

20. The method of claim 18, wherein the culture medium further comprises a distiller'"'"'s grain hydrolysate.

21. The method of claim 20, wherein the hydrolysate includes at least one of an enzymatic hydrolysate or a detoxified acid hydrolysate.

19. (canceled)

22. (canceled)

23. (canceled)

24. A culture medium for bacterial cellulose production, the medium comprising:

at least one of a maize alcohol fermentation filtrate, a maize alcohol fermentation supernatant, a distiller'"'"'s grain filtrate, a distiller'"'"'s grain supernatant, a distiller'"'"'s grain hydrolysate, or a detoxified distiller'"'"'s grain hydrolysate;

a carbon source;

a nitrogen source; and

a salt source.

28. The culture medium of claim 24, wherein the carbon source comprises sugars of hydrolyzed lignocellulose.

29. The culture medium of claim 24, wherein the carbon source is at least one of cellulose or hemicellulose.

30. The culture medium of claim 24, wherein the carbon source comprises a sugar.

31. The culture medium of claim 30, wherein the carbon source is present at about 0.1 to about 20% (w/v) of the amount of at least one of the maize alcohol fermentation filtrate, the maize alcohol fermentation supernatant, a distiller'"'"'s grain filtrate, a distiller'"'"'s grain supernatant, the distiller'"'"'s grain hydrolysate, or the detoxified distiller'"'"'s grain hydrolysate.

33. The culture medium of claim 24 comprising detoxified distiller'"'"'s grain hydrolysate, wherein the carbon source comprises a sugar that is present in an amount of about 0.1 to about 20 percent (w/v) the amount of the detoxified distiller'"'"'s grain hydrolysate, and wherein the nitrogen source is present at about 0.1 to about 2% (w/v) of the amount of the detoxified distiller'"'"'s grain hydrolysate.

25. (canceled)

26. (canceled)

27. (canceled)

32. (canceled)