MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
First Claim
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1. A non-naturally occurring microbial organism, said microbial organism having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein said microbial organism comprises a genetic modification, said genetic modification selected from:
- (A) a genetic modification that increases expression of phosphoenolpyruvate carboxylase;
(B) a genetic modification that increases expression of alpha-ketoglutarate dehydrogenase;
(C) a genetic modification that increases expression of a non-phosphotransferase (PTS) glucose uptake system;
(D) a genetic modification that increases expression of a gamma-butyrolactone esterase;
(E) a genetic modification that decreases expression of succinyl-CoA synthetase;
(F) a genetic modification that decreases expression of an acyl coenzyme A thioesterase;
(G) a genetic modification that decreases expression of an alcohol dehydrogenase(H) a genetic modification that decreases expression of a non-energy-producing NADH dehydrogenase;
(I) a genetic modification that decreases expression of a cytochrome oxidase; and
(J) a combination of two or more of the genetic modifications of parts (A)-(I).
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Abstract
The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.
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6 Claims
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1. A non-naturally occurring microbial organism, said microbial organism having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein said microbial organism comprises a genetic modification, said genetic modification selected from:
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(A) a genetic modification that increases expression of phosphoenolpyruvate carboxylase; (B) a genetic modification that increases expression of alpha-ketoglutarate dehydrogenase; (C) a genetic modification that increases expression of a non-phosphotransferase (PTS) glucose uptake system; (D) a genetic modification that increases expression of a gamma-butyrolactone esterase; (E) a genetic modification that decreases expression of succinyl-CoA synthetase; (F) a genetic modification that decreases expression of an acyl coenzyme A thioesterase; (G) a genetic modification that decreases expression of an alcohol dehydrogenase (H) a genetic modification that decreases expression of a non-energy-producing NADH dehydrogenase; (I) a genetic modification that decreases expression of a cytochrome oxidase; and (J) a combination of two or more of the genetic modifications of parts (A)-(I). - View Dependent Claims (2, 3, 4, 5, 6)
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