FLUID DELIVERY SYSTEM AND METHOD

• US 20140038167A1
• Filed: 01/31/2013
• Published: 02/06/2014
• Est. Priority Date: 01/26/2004
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: SIA, SAMUEL K., LINDER, VINCENT, WHITESIDES, GEORGE M.
• Granted Patent: US 9,116,148 B2
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 137/0318 Processes

Abstract

A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.

Claims

1. A method comprising:

providing a first fluid and a second fluid maintained separately from each other by a third fluid in a common, sealed vessel, wherein the third fluid is adjacent to and substantially immiscible with the first and second fluids;

unsealing the vessel;

transferring the first, third and second fluids in series from the vessel to an analysis region; and

avoiding contact between at least portions of the first and second fluids, at least until after the fluids have been applied to the analysis region.

4. The method of claim 1, further comprising connecting the vessel to a device comprising the analysis region.

5. The method of claim 1, wherein the vessel and analysis region are on a common platform.

6. The method of claim 1, wherein the vessel and analysis region are integrally connected during storage of the first, second and third fluids in the vessel.

7. The method of claim 1, wherein the vessel comprises a tube.

8. The method of claim 1, further comprising applying a pressure differential across the analysis region.

9. The method of claim 8, wherein the pressure differential is provided by suction on a downstream side of the analysis region.

10. The method of claim 8, wherein the pressure differential is provided by a pump on an upstream side of the analysis region.

11. The method of claim 1, wherein the first and second fluids are transferred in series to the analysis region without actuating a valve.

12. The method of claim 1, wherein the first and second fluids are transferred in series to the analysis region without actuation of any device that controls the rate, the order, or timing of introduction of either of the first and second fluids, relative to each other, to the analysis region.

13. The method of claim 4, wherein the device is a microfluidic device.

14. The method of claim 1, wherein at least one of an antibody or an antigen is associated with the analysis region.

15. The method of claim 1, wherein the third fluid is a gas or a gaseous mixture.

16. The method of claim 1, wherein the first fluid and/or second fluid is a rinse solution.

17. The method of claim 1, wherein the analysis region is a part of a device, the method comprising disposing a sample in the device prior to applying the first and second fluids to the analysis region.

18. The method of claim 1, wherein the vessel contains a fourth fluid, the method further comprising combining the fourth fluid and the second fluid while transferring the first, third, and second fluids from the vessel to the analysis region.

19. The method of claim 1, wherein the vessel has a length to inner diameter ratio of at least 10:

1.

20. The method of claim 1, wherein the vessel has an inner diameter of less than 1 millimeter.

21. The method of claim 1, wherein the vessel has an inner diameter of less than 500 microns.

22. The method of claim 1, wherein at least one of the fluids comprises a gold conjugated antibody.

23. The method of claim 1, further comprising electrolessly depositing metal at the analysis region to produce an opaque material.

24. The method of claim 23, wherein at least one of the fluids comprises a metal precursor.

25. The method of claim 23, further comprising determining light absorbance or transmission through the opaque material.

26. The method of claim 1, wherein the first and second fluids are maintained separately from each other by the third fluid in the common, sealed vessel for greater than one hour.

27. The method of claim 1, wherein the analysis region is adapted for allowing a binding event to occur between at least two binding partners, and wherein at least one of the binding partners comprises an antibody.

28. The method of claim 18, wherein the fourth fluid is stored in the vessel prior to the unsealing step.

2. A method comprising:

providing an analysis region;

providing a first fluid and a second fluid maintained separately from each other by a third fluid adjacent to and substantially immiscible with the first and second fluids in a common, sealed vessel, wherein the vessel and the analysis region are formed in a microfluidic chip;

unsealing the vessel;

applying in series the first, third, and second fluids to the analysis region; and

avoiding contact between at least portions of the first and second fluids, at least until after the fluids have been applied to the analysis region.

3. A fluidic system comprising:

an analysis region; and

a sealed vessel containing a first fluid, a second fluid, and a third fluid, wherein the third fluid is adjacent to and substantially immiscible with the first and second fluids,

wherein the sealed vessel is adapted and arranged to be unsealed such that fluids from the vessel can be applied to the analysis region, and

wherein the analysis region and the vessel are formed in a microfluidic chip.