METHODS FOR CULTURING STEM AND PROGENITOR CELLS
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Abstract
The present application describes a method of culturing, expanding or growing stem or stem-like cells or induced pluripotent stem cells on a surface, including attaching the cells to the surface through a ligand that binds to the surface and the cells.
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Citations
47 Claims
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1-23. -23. (canceled)
- 26. A method of identifying state of differentiation of cells comprising using anti-MUC1* antibody to bind to the cells, wherein positive signal for anti-MUC1* antibody indicates puripotent cell state, and cells showing binding to non-clipped MUC1 indicates differentiated cell state.
- 30. A method of detecting cancer stem cells using anti-MUC1* antibody to bind to the cells, wherein positive signal for anti-MUC1* antibody indicates cancer stem cells.
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32-34. -34. (canceled)
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35. A method of separating cell types, comprising
creating a spatial address on a surface for a variety of ligands having affinity for a different cell type or for specific markers that identify a cell stage or type; - and
adding the cells to the surface, wherein the cells are spatially separated depending on which ligand the cells bind. - View Dependent Claims (36, 37)
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- 38. A method of implanting into a host body, a surface having ligands attached that are ligands of stem or stem-like cells or induced pluripotent stem cells.
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41. A method of implanting into a host body, a surface having cells attached through ligands that bind to the surface and the cells.
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42. A method of proliferating stem or stem-like cells or induced pluripotent stem cells in vivo, comprising administering to a host body a surface having ligands attached that are ligands of stem or stem-like cells or induced pluripotent stem cells.
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43. A composition comprising:
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a surface on which is bound protein A or protein G via an affinity interaction, wherein the protein A or protein G is bound to an antibody specific for a polypeptide expressed specifically on a stem or stem-like cell or induced pluripotent stem cell, and a MUC1* dimerizing agent. - View Dependent Claims (44, 45, 46)
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47. A method of proliferating stem or stem-like or induced pluripotent stem cell, comprising:
interacting a surface on which is bound protein A or protein G via an affinity interaction, wherein the protein A or protein G is bound to an antibody specific for a polypeptide expressed specifically on a stem or stem-like cell or induced pluripotent stem cell, with a sample containing the cells with a MUC1* dimerizing agent.
Specification