THREE DIMENSIONAL CELL CULTURE COMPOSITIONS AND METHODS OF USE
First Claim
1. A three-dimensional cell culture scaffold composition comprising a rigid absorbent component and cells, wherein the rigid absorbent component has a void volume of between approximately 70% and 95%, which void volume comprises one or more cells.
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Abstract
Described herein is a three-dimensional cell culture scaffold composition comprising an absorbent rigid (AR) component, and in some embodiments, further comprises a gel component. The absorbent rigid component preferably comprises a glass fiber material. It is a surprising finding of the present invention that an AR component having a void volume of between approximately 70% and 95% results in a three-dimensional cell culture composition that allows for robust, high-throughput screening and high-content screening accessible tissue models with preserved cell morphology, heterogeneity of cell types and cell populations, extracellular matrix constituents, functional cell-cell and cell-extracellular matrix interactions and signaling with sufficient specificities to tissue physiology and pathology.
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Citations
29 Claims
- 1. A three-dimensional cell culture scaffold composition comprising a rigid absorbent component and cells, wherein the rigid absorbent component has a void volume of between approximately 70% and 95%, which void volume comprises one or more cells.
- 13. A method of culturing or suspending cells comprising placing the cells in a three-dimensional cell culture scaffold composition, which composition comprises a rigid absorbent component and has a void volume of between approximately 70% and 95%, thereby creating a three dimensional cell culture or suspension.
- 27. A method of adding one or more molecules comprising placing the one or more molecules in a rigid absorbent component which has a void volume of between approximately 70% and 95%, thereby creating a uniform or non-uniform concentration of the one or more molecules within the rigid absorbent component.
Specification