CELL ANALYSIS METHOD, CELL ANALYSIS DEVICE, AND CELL ANALYSIS PROGRAM

• US 20140064594A1
• Filed: 02/23/2012
• Published: 03/06/2014
• Est. Priority Date: 04/28/2011
• Status: Active Grant

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Sugiyama, Norikazu, Takeshima, Tomochika, Kaneko, Kouichi
• Granted Patent: US 9,405,958 B2
• Time in Patent Office: Days
• US Class Current: 382/133
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 33/5026 on cell morphology
• CPC Class Codes: G02B 21/14 affording illumination for phase-contrast observation
• CPC Class Codes: G06V 20/693 Acquisition
• CPC Class Codes: G06V 20/695 Preprocessing, e.g. image segmentation
• CPC Class Codes: G06V 20/698 Matching; Classification

Abstract

Provided is a cell analysis method in a cell analysis device D that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony, the method comprising: acquiring the optical path length image of the cell colony by an acquisition unit of the cell analysis device; extracting a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image by an extraction unit of the cell analysis device extracts; comparing an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape by a comparison unit of the cell analysis device extracts; and analyzing the cell colony based on the comparison result by analysis unit of the cell analysis device.

Claims

1. :

A cell analysis method in a cell analysis device that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony, the method comprising;

acquiring the optical path length image of the cell colony by an acquisition unit of the cell analysis device;

extracting a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image by an extraction unit of the cell analysis device extracts;

comparing an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape by a comparison unit of the cell analysis device extracts; and

analyzing the cell colony based on the comparison result by analysis unit of the cell analysis device.

2. :

The cell analysis method according to claim 1, wherein in the comparing and the analyzing, when the outer optical path length of the circular shape is larger than the inner optical path length of the circular shape, the cell is determined as a cell with good quality.

3. :

The cell analysis method according to claim 2, wherein in the analyzing, when the number of cells per unit, which are determined as the cell with good quality, is equal to or greater than a threshold, the cell colony is determined as a cell colony with good quality.

4. :

The cell analysis method according to claim 3, further comprising;

specifying and displaying a region of the cell colony with good quality by display unit of the cell analysis device.

5. :

The cell analysis method according to claim 1, wherein the optical path length image is a quantitative optical path length image.

6. :

The cell analysis method according to claim 1, wherein the circular shape is a true circular shape.

7. :

The cell analysis method according to claim 6, wherein the true circular shape is a true circular shape having a predetermined diameter range.

8. :

The cell analysis method according to claim 1, wherein the circular shape is an elliptical shape.

9. :

The cell analysis method according to claim 1, wherein the cell is a stem cell.

10. :

A cell analysis device that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony, the device comprising;

acquisition unit configured to acquire the optical path length image of the cell colony;

extraction unit configured to extract a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image;

comparison unit configured to compare an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape; and

analysis unit configured to analyze the cell colony based on the comparison result.

11. :

A cell analysis program that causes a computer to function as;

in a cell analysis device that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony,

acquisition unit configured to acquire the optical path length image of the cell colony;

extraction unit configured to extract a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image;

comparison unit configured to compare an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape; and

analysis unit configured to analyze the cell colony based on the comparison result.