BRASSICA OLERACEA PLANTS WITH IMPROVED NUTRITIONAL VALUE

• US 20140075590A1
• Filed: 09/12/2013
• Published: 03/13/2014
• Est. Priority Date: 09/13/2012
• Status: Active Grant

• Current Assignee: Plant Bioscience Limited (Alnylam Pharmaceuticals Incorporated), Seminis Vegetable Seeds Incorporated (Bayer AG)
• Original Assignee: Plant Bioscience Limited (Alnylam Pharmaceuticals Incorporated), Seminis Vegetable Seeds Incorporated (Bayer AG)
• Inventors: Koorevaar, Gerard N., Mithen, Richard F., VAN DEN BOSCH, FRANCISCUS G.
• Granted Patent: US 9,617,554 B2
• Time in Patent Office: Days
• US Class Current: 800/263
• CPC Class Codes: A01H 1/02 Methods or apparatus for hybridisation; Artificial pollination ; Fertility
• CPC Class Codes: A01H 1/04 Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
• CPC Class Codes: A01H 5/02 Flowers
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/203 Brassica oleraceae, e.g. broccoli or kohlrabi
• CPC Class Codes: C12N 15/8246 Non-starch polysaccharides, e.g. cellulose, fructans, levans
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

Claims

1. A Brassica oleracea plant comprising a Myb28 allele from Brassica villosa and lacking an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein the Myb28 allele confers elevated glucosinolates when compared to a plant that lacks the Myb28 allele.

2. The plant of claim 1, wherein the plant is a broccoli plant.

3. The plant of claim 1, wherein the plant is inbred.

4. The plant of claim 1, wherein the plant is hybrid.

5. The plant of claim 1, wherein the plant is homozygous for said Myb28 allele from Brassica villosa.

6. The plant of claim 1, wherein the plant is heterozygous for said Myb28 allele from Brassica villosa.

7. The plant of claim 1, wherein the ELONG allele is from Brassica oleracea.

8. A plant part of the plant of claim 1.

9. The plant part of claim 8, wherein the part is a leaf, a ovule, a floret, pollen, a head, or a cell.

10. A seed that produces the plant of claim 1.

11. A Brassica oleracea plant comprising a chromosomal segment that comprises a Myb28 allele from Brassica villosa and lacking an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein the segment confers elevated glucosinolates relative to a plant lacking the Myb28 allele, wherein a sample of seed comprising the chromosomal segment was deposited under ATCC Accession Number PTA-13165.

12. A seed that produces the plant of claim 11.

13. A plant part of the plant of claim 11.

14. The plant part of claim 13, wherein the part is a leaf, an ovule, a floret, pollen, a head, or a cell.

15. A recombined DNA segment comprising a Myb28 allele from Brassica villosa and an ELONG allele from Brassica oleracea.

16. The DNA segment of claim 15, further defined as comprised within a cell.

17. The DNA segment of claim 15, further defined as comprised within a seed.

18. The DNA segment of claim 15, further defined as comprised within a plant.

19. A method for obtaining a Brassica plant comprising a desired glucosinolate composition comprising:

(a) obtaining a Brassica plant heterozygous for a Myb28 allele from Brassica villosa that confers elevated glucosinolates and is genetically linked in the plant to an ELONG allele from Brassica villosa;

(b) obtaining progeny of the plant; and

(c) selecting at least a first progeny plant in which recombination has occurred such that the progeny comprises the Myb28 allele but not the ELONG allele from Brassica villosa, wherein the progeny plant possesses a desired glucosinolate composition as a result of the presence of the Myb28 allele but not the ELONG allele from Brassica villosa.

20. The method of claim 19, wherein selecting the progeny plant comprises identifying a progeny plant that (1) comprises a genetic marker genetically linked to the Myb28 allele in Brassica villosa and/or lacks a genetic marker present at the corresponding locus in said Brassica plant, and (2) lacks a genetic marker genetically linked to the ELONG allele from Brassica villosa and/or comprises a genetic marker present at the corresponding locus from said Brassica plant.

21. The method of claim 20, wherein selecting a progeny plant comprises detecting a polymorphism that is found in the genome of said plant flanked by the complements of SEQ ID NO:

1 and SEQ ID NO;

2.

22. The method of claim 21, wherein (a) the allele(s) are detected by a PCR-based method using oligonucleotide primer pair(s).

23. The method of claim 20, wherein selecting a progeny plant comprises detecting a polymorphism in said progeny plant that is shown in FIG. 5.

24. The method of claim 19, wherein Brassica plant is a B. oleracea plant.

25. A plant produced by the method of claim 19 or a progeny thereof comprising said Myb28 allele but not the ELONG allele from Brassica villosa.

26. A part of the plant of claim 25 selected from the group consisting of a cell, a seed, a root, a stem, a leaf, a head, a flower, and pollen.

27. A method for producing a hybrid Brassica oleracea plant with elevated glucosinolate content comprising crossing a first Brassica oleracea parent plant with a second Brassica oleracea plant of a different genotype, wherein the first parent plant comprises a Myb28 allele from Brassica villosa that lacks an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein the Myb28 allele confers elevated glucosinolates relative to a plant lacking the Myb28 allele.

28. The method of claim 27, further comprising producing a plurality of hybrid Brassica oleracea plants comprising crossing the first Brassica oleracea parent plant with a plurality of second Brassica oleracea plants of different genotypes.

29. A method of producing a Brassica oleracea plant with a desired elevated glucosinolate content comprising introgressing into the plant a chromosomal segment comprising a Myb28 allele from Brassica villosa and lacking an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein segment confers a desired glucosinolate content relative to a plant lacking the segment, wherein a sample of seed comprising the chromosomal segment is deposited under ATCC Accession No. PTA-13165.