Plant Hormone Biosensors
First Claim
1. A fusion protein comprisinga) at least a first and second fluorescent protein, wherein the first and second fluorescent proteins emit wavelengths of light that are different from one anotherb) at least one plant hormone binding domain comprising an N-terminus and a C-terminus, wherein the binding domain changes three-dimensional conformation upon specifically binding to a plant hormone, andc) at least a first and second fluorescent protein linker peptide, wherein the first fluorescent protein linker peptide links the first fluorescent protein to the N-terminus of the at least one plant hormone binding domain and the second fluorescent protein linker peptide links the second fluorescent protein to the C-terminus of the at least one plant hormone binding domain.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention provides fusion proteins comprising at least two fluorescent proteins, with the fluorescent proteins emitting different wavelengths of light from one another, at least one plant hormone binding domain that changes three-dimensional conformation upon specifically binding to a plant hormone, and two linker peptides, with the first linker linking the first fluorescent protein to the N-terminus of the plant hormone binding domain and the second linker linking the second fluorescent protein to the C-terminus of the plant hormone binding domain. The invention also provides for methods of using the fusion proteins of the present invention and nucleic acids encoding the fusion proteins.
-
Citations
20 Claims
-
1. A fusion protein comprising
a) at least a first and second fluorescent protein, wherein the first and second fluorescent proteins emit wavelengths of light that are different from one another b) at least one plant hormone binding domain comprising an N-terminus and a C-terminus, wherein the binding domain changes three-dimensional conformation upon specifically binding to a plant hormone, and c) at least a first and second fluorescent protein linker peptide, wherein the first fluorescent protein linker peptide links the first fluorescent protein to the N-terminus of the at least one plant hormone binding domain and the second fluorescent protein linker peptide links the second fluorescent protein to the C-terminus of the at least one plant hormone binding domain.
-
17. A method of producing a fusion protein, the method comprising culturing a host cell in conditions that promote protein expression and recovering the fusion protein from the culture, wherein the host cell comprises a vector encoding a fusion protein, wherein the fusion protein comprises
a) at least a first and second fluorescent protein, wherein the first and second fluorescent proteins emit wavelengths of light that are different from one another b) at least one plant hormone binding domain comprising an N-terminus and a C-terminus, wherein the binding domain changes three-dimensional conformation upon specifically binding to a plant hormone, and c) at least a first and second fluorescent protein linker peptide, wherein the first fluorescent protein linker peptide links the first fluorescent protein to the N-terminus of the at least one plant hormone binding domain and the second fluorescent protein linker peptide links the second fluorescent protein to the C-terminus of the at least one plant hormone binding domain.
Specification