FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS
First Claim
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1. A method for integrating a nucleic acid sequence of interest into a specific site in the genome of a soybean cell, the method comprising:
- cleaving, in a site specific manner, a target site in a FAD2 gene in a soybean cell, to thereby generate a break in the FAD2 gene;
integrating into the break a nucleic acid sequence of interest.
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Abstract
Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
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Citations
36 Claims
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1. A method for integrating a nucleic acid sequence of interest into a specific site in the genome of a soybean cell, the method comprising:
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cleaving, in a site specific manner, a target site in a FAD2 gene in a soybean cell, to thereby generate a break in the FAD2 gene; integrating into the break a nucleic acid sequence of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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- 18. A transgenic soybean cell comprising a nucleotide sequence of interest integrated into a FAD2 2.3 and/or FAD2 2.6 gene.
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26. A site specific zinc finger nuclease that cleaves at or near a nucleic acid target site selected from the group consisting of SEQ ID NO:
- 14 to SEQ ID NO;
20. - View Dependent Claims (27)
- 14 to SEQ ID NO;
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28. A method of cleaving a FAD2 loci in a soybean cell, the method comprising:
introducing a fusion protein comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the fusion protein binds with specificity to a target site in the FAD2 loci and cleaves at or near the target site to thereby generate a break. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36)
Specification