CONTROL OF INSECT PESTS THROUGH RNAI OF PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE RECEPTOR
First Claim
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1. A double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a pheromone-biosynthesis-activating neuropeptide receptor in a cell, wherein said dsRNA comprises a sense strand comprising a first sequence and an antisense strand comprising a second sequence complementary to SEQ. ID. NO. 2, wherein said first sequence is complementary to said second sequence.
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Abstract
Disclosed are dsRNA constructs and methods to control insects via double stranded RNA interference of insect PBAN receptor genes.
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19 Claims
- 1. A double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a pheromone-biosynthesis-activating neuropeptide receptor in a cell, wherein said dsRNA comprises a sense strand comprising a first sequence and an antisense strand comprising a second sequence complementary to SEQ. ID. NO. 2, wherein said first sequence is complementary to said second sequence.
- 3. A double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a pheromone-biosynthesis-activating neuropeptide receptor in a cell, wherein said dsRNA comprises a sense strand comprising a first sequence and an antisense strand comprising a second sequence complementary to SEQ. ID. NO. 4, wherein said first sequence is complementary to said second sequence.
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5. A method for controlling an insect, the method comprising:
- constructing a double stranded ribonucleic acid construct that is complementary to a gene that encodes an PBAN receptor gene sequence, dissolving the double stranded ribonucleic acid to form a solution, and contacting an effective amount of said solution to insect, wherein RNA interference is induced and insect control occurs.
- View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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