ENHANCED OXYGEN CELL CULTURE PLATFORMS
1 Assignment
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Accused Products
Abstract
Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of the culture vessel, top and bottom. A gas-permeable membrane that includes perfluorocarbons or fluorocarbon derivatives (e.g., fluorinated silane, partially fluorinated silane) in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which the cell culture apparatus is incubated.
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Citations
67 Claims
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1-48. -48. (canceled)
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49. A cell and tissue culture apparatus comprising:
a tissue culture device containing a gas permeable, liquid impermeable membrane barrier, the gas permeable, liquid impermeable membrane comprising a silicone composition impregnated, mixed or combined with a fluorocarbon derivative;
support members extending from the bottom, side or top of the tissue culture device to elevate the tissue culture device to allow air flow; and
, a tray or culture flask comprising the tissue culture device, wherein the liquid impermeable membrane barrier defines a continuous surface of the tissue culture device and allows oxygenation of and minimization of hypoxia in cells resting thereupon.- View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 64, 65, 66, 67)
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63. An apparatus for the transportation of cells, tissues and organs comprising:
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a culture device containing a gas permeable, liquid impermeable membrane barrier to allow air flow, the gas permeable, liquid impermeable membrane comprising a silicone composition impregnated, mixed or combined with a fluorocarbon derivative, wherein the liquid impermeable membrane barrier defines a continuous surface of at least one side of the culture device and allows oxygenation of and minimization of hypoxia in cells resting thereupon; and
,a container enclosing the culture device.
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Specification