MULTIWELL PLATE AND METHOD OF ANALYZING TARGET MATERIAL USING THE SAME

• US 20140106394A1
• Filed: 10/16/2013
• Published: 04/17/2014
• Est. Priority Date: 10/16/2012
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: KO, Eun, Lee, Moon-sook
• Application Number:
  US14/055,360
• Publication Number:
  US 20140106394A1
• Time in Patent Office: Days
• US Class Current: 435/30
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0854 Double walls
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 25/04 in combination with well or multiwell plates, i.e. culture inserts
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Abstract

Provided is a multiwell plate for an easy liquid removal, wherein the mutiwell plate includes a well with at least one pore in the sidewall defining the well, and a method of analyzing a target material using the multiwell plate.

Claims

1. A multiwell plate comprising at least one first well defined by a bottom wall and a side wall connected to the bottom wall along the periphery of the bottom wall, wherein the well comprises at least one pore in the side wall.

2. A multiwell plate set comprising:

a first multiwell plate according to claim 1; and

a second multiwell plate comprising a second well having a dimension that accommodates the first well of the first multiwell plate, such that the first well of the first multiwell plate can be inserted into the second well of the second multiwell plate.

3. The first multiwell plate of claim 1, wherein the sidewall has an edge opposite the bottom wall that defines a top opening, and wherein the top opening is wider than the bottom wall width.

4. The first multiwell plate of claim 1, wherein the pore is smaller than a spheroid, a tissue, an organ, or a cell.

5. The first multiwell plate of claim 1, wherein the side wall of the first well does not contact a side wall of another well of the first multiwell plate.

6. The first multiwell plate of claim 1, wherein the multiwall plate comprises an array of first wells.

7. The first multiwell plate of claim 1, wherein the pore is located in the sidewall about 0.1 to about 5 mm from the bottom wall.

8. The first multiwell plate of claim 1, wherein a narrowest part of the bottom wall has a width about 0.5 to about 1 mm

9. The first multiwell plate of claim 4, wherein a narrowest part of the pore has a width about 50 to about 100 μ

m.

10. The first multiwell plate of claim 1, wherein the bottom is flat or concave and protrudes away from the interior of the well.

11. The first multiwell plate of the claim 1, wherein sidewall has an edge opposite the bottom wall that defines a top opening, and the top opening is separated from the bottom wall by a distance of about 5 to about 15 mm.

12. The first multiwell plate of claim 1, wherein the bottom wall has an interior surface facing the interior of the well, and the interior surface of the bottom is coated with a coating material.

13. The first multiwell plate of claim 1, wherein the first multiwell plate further comprises a lid covering the top opening of the first well.

14. The multiwell plate set of claim 2, wherein the second multiwell plate includes a chamber beneath the second well, wherein the chamber comprises a bottom and a side wall, and the top surface of the chamber is defined by the bottom of the second well.

15. The multiwell plate set of claim 14, wherein the bottom of the second well is located between a pair of electrodes, wherein one electrode is located at the top of the second well while the other electrode is located at the bottom of the chamber.

16. The multiwell plate set of claim 14, wherein the bottom of the chamber, the side wall of the chamber, or both are movable and can be opened and closed.

17. A method of analyzing a target material, comprising:

introducing a sample including a target material into the first well of the first multiwell plate of claim 1; and

inserting the first well of the first multiwell plate into a second well of a second multiwell plate.

18. The method of claim 17, wherein the method further comprises inflowing a liquid into the second well of the second multiwell plate.

19. The method of claim 17, further comprising moving the first multiwell plate such that the liquid penetrates through the at least one pore of the first well, wherein the target material cannot penetrate the pore.

20. The method of claim 17, further comprising centrifuging the first multiwell plate, to form a spheroid.