ALLERGEN TESTING PLATFORM FOR USE WITH MOBILE ELECTRONIC DEVICES

• US 20140120563A1
• Filed: 10/14/2013
• Published: 05/01/2014
• Est. Priority Date: 10/29/2012
• Status: Abandoned Application

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Ozcan, Aydogan, Coskun, Ahmet F., Wong, Justin
• Application Number:
  US14/053,475
• Publication Number:
  US 20140120563A1
• Time in Patent Office: Days
• US Class Current: 435/7.94
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

An allergy testing system for use with a mobile electronic device having a camera includes a housing that can be attached to the mobile electronic device. First and second light sources within the housing are configured to illuminate, respectively, a test sample and a control sample. A colorimetric assay is performed on the test sample and the control sample. The first light source and the second light source are activated and the camera of the mobile electronic device captures images of transmitted light. The relative intensity of transmitted light is then used by software loaded on the mobile electronic device to determine a relative absorbance value. The relative absorbance value is used, together with a calibration curve, to measure the concentration of a particular allergen within the test sample. Based on the concentration of the allergen the test sample can be labeled as either “positive” or “negative.”

Claims

1. An allergy testing system for use with a mobile electronic device having a camera comprising:

a housing configured for detachable attachment to the mobile electronic device at a location adjacent to the camera;

a first light source disposed within the housing and configured to illuminate a control holder containing a control sample therein;

a second light source disposed within the housing and configured to illuminate a sample holder containing a test sample therein;

first and second apertures disposed adjacent to the control holder and sample holder, respectively, wherein the control holder and sample holder is interposed between the first and second apertures and the first and second light sources; and

a lens disposed in the housing and interposed between the camera and the first and second apertures.

2. The allergy testing system of claim 1, further comprising a diffuser interposed between the first light source and the control holder and a diffuser interposed between the second light source and the control holder.

3. The allergy testing system of claim 1, further comprising a power source disposed in the housing and configured to power the first light source and the second light source.

4. The allergy testing system of claim 1, wherein the first light source and the second light source are coupled to a power source contained in the mobile electronic device.

5. The allergy testing system of claim 1, wherein the control holder and the sample holder comprise bound antibodies as part of a sandwich ELISA assay.

6. The allergy testing system of claim 5, wherein the sandwich ELISA assay is specific for an allergen selected from the group comprising nuts, casein, crustacean allergen, eggs, gliadins, gluten, lupin, mustard, soy, wheat, sesame, and milk.

7. The allergy testing system of claim 1, wherein the mobile electronic device comprises software loaded thereon that is configured to calculate the relative intensity of light of the control sample and test sample received at the camera.

8. The allergy testing system of claim 7, wherein the software is configured to output the measured concentration of an allergen in the test sample.

9. The allergy testing system of claim 8, wherein the software is configured to output a “

positive”

or “

negative”

reading of the test sample based at least in part on a comparison of the measured concentration with a threshold value.

10. The allergy testing system of claim 5, further comprising an allergen testing kit comprising:

an allergen extractor;

one or more control samples having a known concentration of allergen; and

a plurality of sandwich ELISA reagents.

11. The allergy testing system of claim 10, wherein the light sources comprise LEDs or laser diodes.

12. The allergy testing system of claim 1, further comprising a stand configured to hold the mobile electronic device in a substantially vertical orientation.

13. The allergy testing system of claim 1, wherein the control holder comprises multiple control samples and wherein the sample holder comprises multiple test samples.

14. A method of testing an article of food for an allergen comprising:

exposing the article of food to an extraction solution;

transferring a portion of the extraction solution exposed to the article of food to a sample holder comprising a sandwich ELISA assay;

transferring a control sample having a known concentration of allergen to a control holder comprising a sandwich ELISA assay;

emptying the sample holder and control holder;

washing the sample holder and control holder with a wash buffer;

exposing the sample holder and control holder to a first sandwich ELISA reagent;

washing the sample holder and control holder with a wash buffer;

exposing the sample holder and control holder to a second sandwich ELISA reagent;

washing the sample holder and control holder with a wash buffer;

exposing the sample holder and control holder to a third sandwich ELISA reagent; and

inserting the sample holder and control holder into the housing of the system of claim 1; and

powering the first and second light sources;

measuring the relative intensities of light passing through the sample holder and control holder; and

calculating a concentration of the allergen based at least in part on the measured relative intensities of light passing through the sample holder and the control holder.

15. A method of testing an article of food for an allergen using a mobile electronic device having a camera therein comprising:

subjecting a test sample containing the article of food and a control sample containing a known quantity of allergen to a colorimetric assay;

illuminating the test sample and the control sample with first and second illumination sources;

capturing at least a portion of the transmitted illumination light through the test sample and the control sample with the camera of the mobile electronic device;

calculating the relative intensity of the transmitted illumination light through the test sample and the control sample using software contained on the mobile electronic device; and

displaying a concentration of the allergen on the mobile electronic device based at least in part on the calculated relative intensity of the transmitted illumination light.

16. The method of claim 15, further comprising displaying on the mobile electronic device an indication of a positive sample or a negative sample based on the concentration of the allergen.

17. The method of claim 15, further comprising transmitting the concentration of the allergen to a remote computer or database.

18. The method of claim 15, wherein the allergen is selected from the group comprising nuts, casein, crustacean allergen, eggs, gliadins, gluten, lupin, mustard, soy, wheat, sesame, and milk.

19. The method of claim 15, wherein the colorimetric assay comprises a sandwich ELISA assay.

20. The method of claim 15, wherein the first and second illumination sources are contained in a housing configured to be detachably mounted to the mobile electronic device.