DRY INSERTION AND ONE-POINT IN VIVO CALIBRATION OF AN OPTICAL ANALYTE SENSOR
First Claim
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1. A method for monitoring glucose concentration in a physiological fluid in a patient, comprising:
- providing a sensor comprising an equilibrium fluorescence chemical indicator immobilized in a hydrogel disposed along a distal region of an optical fiber, wherein the hydrogel comprising the immobilized chemical indicator system is in a dry state;
deploying the distal region of the optical fiber in the physiological fluid, wherein the hydrogel with immobilized chemical indicator system is deployed in a dry state;
allowing the hydrogel with immobilized chemical indicator system to hydrate in the physiological fluid;
performing an in vivo calibration of the chemical indicator system against an independently measured glucose concentration, and optionally pH levels, in the physiological fluid; and
monitoring the glucose concentration in the physiological fluid.
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Abstract
Disclosed are embodiments that relate to the deployment of a glucose sensor comprising an optical fiber into a physiological fluid, wherein the optical fiber has disposed along a distal region thereof a chemical indicator system comprising a fluorophore and a glucose binding moiety immobilized within a hydrogel, wherein the components of the chemical indicator system are in a dry state before deployment. Also disclosed is a one-point in vivo calibration of the chemical indicator system based on an independently measured glucose concentration.
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Citations
12 Claims
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1. A method for monitoring glucose concentration in a physiological fluid in a patient, comprising:
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providing a sensor comprising an equilibrium fluorescence chemical indicator immobilized in a hydrogel disposed along a distal region of an optical fiber, wherein the hydrogel comprising the immobilized chemical indicator system is in a dry state; deploying the distal region of the optical fiber in the physiological fluid, wherein the hydrogel with immobilized chemical indicator system is deployed in a dry state; allowing the hydrogel with immobilized chemical indicator system to hydrate in the physiological fluid; performing an in vivo calibration of the chemical indicator system against an independently measured glucose concentration, and optionally pH levels, in the physiological fluid; and monitoring the glucose concentration in the physiological fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A glucose sensor, comprising an optical fiber comprising an equilibrium fluorescence chemical indicator system immobilized within a hydrogel disposed along a distal end region of the optical fiber, wherein the immobilized chemical indicator system is in a dry state, and an optical coupling disposed along a proximal end region of the optical fiber.
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12. A method for monitoring glucose concentration in a physiological fluid in a patient, comprising:
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providing a sensor comprising an equilibrium fluorescence chemical indicator immobilized in a hydrogel disposed along a distal region of an optical fiber; deploying the distal region of the optical fiber in the physiological fluid without performing ex vivo calibration; performing in vivo calibration of the chemical indicator system against an independently measured glucose concentration in the physiological fluid; and monitoring the glucose concentration in the physiological fluid.
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Specification