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PLURIPOTENT STEM CELL-DERIVED BROWN ADIPOCYTES, PLURIPOTENT STEM CELL-DERIVED CELL AGGREGATE, METHOD FOR PRODUCING SAME, AND CELL THERAPY AND MEDICAL THERAPY THEREFOR

  • US 20140140967A1
  • Filed: 04/26/2012
  • Published: 05/22/2014
  • Est. Priority Date: 04/27/2011
  • Status: Active Grant
First Claim
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1. A method of producing brown adipocytes from pluripotent stem cells, the method comprising:

  • forming cell aggregates from pluripotent stem cells by a method including the step (A); and

    preparing brown adipocytes from the cell aggregates by a method including the step (B);

    (A) production of cell aggregates by non-adhesive culture of pluripotent stem cells in serum-free environment in the presence of one or more hematopoietic cytokines; and

    (B) production of brown adipocytes by adhesion culture of the cell aggregates in the presence of one or more hematopoietic cytokines.

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