NUCLEIC ACID AMPLIFICATION
0 Assignments
0 Petitions
Accused Products
Abstract
In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
-
Citations
37 Claims
-
1-20. -20. (canceled)
-
21. A method for nucleic acid amplification, comprising:
-
(a) forming a reaction mixture including a continuous liquid phase containing a first support, a second support, a first polynucleotide template, a second polynucleotide template, a first universal primer and a polymerase, wherein the first universal primer contains a sequence that is complementary or identical to a corresponding primer-binding sequence present in the first and second polynucleotide templates; and (b) forming two or more substantially monoclonal populations, each linked to a different support, by clonally amplifying, within the continuous liquid phase, the first polynucleotide template on the first support and the second polynucleotide template on the second support, under isothermal amplification conditions. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
-
Specification