Cryogenic Storage Container With Sealing Closure and Methods of Using the Same
First Claim
Patent Images
1. A sealable container comprising:
- a body comprising a proximal open end and a sealed distal end and a variable diameter lumen extending there between, the lumen having an external surface and an internal surface, the internal surface tapering generally radially inwardly from the proximal open end to the distal end and forming a sealing portion and a sample receiving portion, a closure configured for removable attachment to the body wherein the closure comprises a capping member adapted for engagement with the proximal open end and a deformable member having a tapered external surface configured for sealing engagement with the sealing portion of lumen internal surface, at least two projections extending radially outwardly from the external surface of the lumen, the projections comprising a radially outward-most surface extending between the proximal open end and the sealed distal end of the body, wherein the outward-most surface has a substantially constant diameter.
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Abstract
A container or vial for the cryopreservation and/or vitrification and storage of a biological sample, including cellular samples and embryos is described. The container comprises a sealable closure that isolates a biological sample held in the container lumen from the external environment and is suitable for storage of the sample in liquid nitrogen. Methods for introducing and sealing biological samples within the container for cryopreservation and/or vitrification and storage are also described.
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Citations
20 Claims
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1. A sealable container comprising:
a body comprising a proximal open end and a sealed distal end and a variable diameter lumen extending there between, the lumen having an external surface and an internal surface, the internal surface tapering generally radially inwardly from the proximal open end to the distal end and forming a sealing portion and a sample receiving portion, a closure configured for removable attachment to the body wherein the closure comprises a capping member adapted for engagement with the proximal open end and a deformable member having a tapered external surface configured for sealing engagement with the sealing portion of lumen internal surface, at least two projections extending radially outwardly from the external surface of the lumen, the projections comprising a radially outward-most surface extending between the proximal open end and the sealed distal end of the body, wherein the outward-most surface has a substantially constant diameter. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for preparing a biological sample for cryogenic storage comprising:
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a. obtaining sealable container comprising; i. a body comprising a proximal open end and a sealed distal end and a variable diameter lumen extending there between, the lumen having an external surface and an internal surface, the internal surface tapering generally radially inwardly from the proximal open end to the distal end and forming a sealing portion and a sample receiving portion, ii. a closure configured for removable attachment to the body wherein the closure comprises a capping member adapted for engagement with the proximal open end and a deformable member having a tapered external surface configured for sealing engagement with the sealing portion of lumen internal surface, iii. at least two projections extending radially outwardly from the external surface of the lumen, the projections comprising a radially outward-most surface extending between the proximal open end and the sealed distal end of the body, wherein the outward-most surface has a substantially constant diameter; b. introducing a biological sample into the lumen of the container; c. attaching the closure to the container body. - View Dependent Claims (14, 15, 16, 17, 18)
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19. A cryogenic storage method comprising:
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a. introducing a biological sample into the lumen of a container, the container comprising; i. a body comprising a proximal open end and a sealed distal end and a variable diameter lumen extending there between, the lumen having an external surface and an internal surface, the internal surface tapering generally radially inwardly from the proximal open end to the distal end and forming a sealing portion and a sample receiving portion, ii. a closure configured for removable attachment to the body wherein the closure comprises a capping member adapted for engagement with the proximal open end and a deformable member having a tapered external surface configured for sealing engagement with the sealing portion of lumen internal surface, iii. at least two projections extending radially outwardly from the external surface of the lumen, the projections comprising a radially outward-most surface extending between the proximal open end and the sealed distal end of the body, wherein the outward-most surface has a substantially constant diameter; b. exposing at least a portion of the container to a cryogenic freezing medium. - View Dependent Claims (20)
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Specification