SINGLE CHAIN PROTEINS WITH C-TERMINAL MODIFICATIONS
First Claim
1. An isolated VH single domain antibody comprising a C-terminal modification, wherein the C-terminal modification comprises a deletion of at least one amino acid residue such that the deletion of at least one amino acid residue in the single domain antibody eliminates the interaction of a pre-existing antibody with the single domain antibody without interfering with the binding of the single domain antibody with its target.
4 Assignments
0 Petitions
Accused Products
Abstract
The invention pertains to an isolated VH single domain antibody comprising a C-terminal modification, where the C-terminal modification comprises a deletion of at least one amino acid residue that eliminates the interaction of a pre-existing antibody with the single domain antibody without interfering with the binding of the single domain antibody with its target.
-
Citations
25 Claims
- 1. An isolated VH single domain antibody comprising a C-terminal modification, wherein the C-terminal modification comprises a deletion of at least one amino acid residue such that the deletion of at least one amino acid residue in the single domain antibody eliminates the interaction of a pre-existing antibody with the single domain antibody without interfering with the binding of the single domain antibody with its target.
-
22. A method of eliminating a pre-existing immune response in a subject comprising:
- administering a single domain antibody comprising a C-terminal modification, wherein the C-terminal modification comprises a deletion of at least one amino acid residue such that the deletion of at least one amino acid residue to the single domain antibody eliminates the interaction of a pre-existing antibody with the single domain antibody without interfering with the binding of the single domain antibody with its target.
-
23. A method of improving a response to single domain antibody in a subject having a pre-existing antibody against a single domain antibody, comprising:
- administering single domain antibody comprising a C-terminal modification, wherein the C-terminal modification comprises a deletion of at least one amino acid residue such that the deletion of at least one amino acid residue to the single domain antibody eliminates the interaction of a pre-existing antibody with the single domain antibody without interfering with the binding of the single domain antibody with its target.
-
24. A method of predicting whether a pre-existing antibody will produce a pre-existing immune response with a single domain antibody comprising:
-
contacting the single domain antibody with a human sample; determining whether a pre-existing antibody, if present in the human sample, binds to the single domain scaffold; and modifying the C-terminal region of single domain antibody by the deletion of at least one amino acid residue such that the C-terminal modification eliminates the interaction of the pre-existing antibody with the single domain antibody.
-
Specification