TANGENTIAL FLOW FILTRATION APPARATUSES, SYSTEMS, AND PROCESSES FOR THE SEPARATION OF COMPOUNDS
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Abstract
The invention relates to apparatuses, machines, systems and methods for the recovery and purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds from aqueous fluids. The aqueous fluids can comprise enzyme concentrates and or a fermentation broth with or without cells or other starting material. The fermentation broth can be produced by fermentations of fungal, yeast, bacterial, mammalian, insect or plant cells.
23 Citations
69 Claims
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1-51. -51. (canceled)
- 52. A filtration system comprising a plurality of fluidly-interconnected filtration modules including a first module and multiple subsequent modules, wherein each module is configured to route received feed material and diluent adjacent a filter to provide permeate and retentate, the first module receiving feed at an inlet side from outside the system and the subsequent modules receiving retentate from a preceding module within the system as feed material, at least one of the modules having a permeate withdrawal flow line for withdrawing permeate from the system, and a plurality of the modules having a return permeate exit configured for feeding permeate back to an inlet side of a same or a preceding module within the system.
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55. A filtration system comprising:
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a system inlet for receiving fresh feed from outside the system, a plurality of fluidly-interconnected filtration modules comprising a first module and multiple subsequent modules, wherein the first module comprises; an inlet side in fluid communication with the system inlet for receiving fresh feed, a diafiltration line for introducing diluent to the inlet side, a filter for selectively separating a species of interest from feed material comprising a mixture of fresh feed and diluent for providing permeate that passes through the filter to an outlet side of the module and retentate that does not pass through the filter, at least one permeate flow line for withdrawing permeate from the outlet side of the first module and optionally another permeate flow line for recycling permeate to the inlet side of the first module, and a retentate flow line configured to route retentate flow from the inlet side of the module to the inlet side of a subsequent module, wherein the subsequent modules each comprise; an inlet side in fluid communication with a retentate flow line of a preceding module, a diafiltration line for introducing diluent to the inlet side, a filter for selectively separating the species of interest from feed material introduced at the inlet side thereof, a permeate flow line configured for returning permeate flow from the outlet side of the module to the inlet side of the same module or for backfeeding permeate flow to a preceding module, another permeate flow line configured for withdrawing permeate from the system, and a retentate flow line configured to route retentate from the inlet side of the module to the inlet side of a subsequent module or otherwise discharge the retentate for recovery or recirculation within the system. - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
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Specification