Gleason Grading by Segmenting and Combining Co-Registered Images of Differently Stained Tissue Slices
First Claim
1. A method comprising:
- generating first objects from a first digital image of a first tissue slice, wherein the first tissue slice has been stained with a first stain that stains basal epithelial cells;
generating second objects from a second digital image of a second tissue slice, wherein the second tissue slice has been stained with a second stain that stains luminal epithelial cells;
co-registering the first digital image with the second digital image;
defining third objects that include only those second objects that have more than a minimum separation from any of the first objects;
defining a scoring region that includes the third objects; and
determining a histopathological score in the scoring region.
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Abstract
An improved histopathological score is obtained by generating image objects from images of tissue containing stained epithelial cells. First objects are generated that correspond to basal cells stained with a first stain, such as p63. Second objects are generated that correspond to luminal cells stained with a second stain, such as CK18. If the same tissue is not stained with both stains, then the images of differently stained tissue are co-registered. Third objects are defined to include only those second objects that have more than a minimum separation from any first object. A scoring region includes the third objects, and the histopathological score is determined based on tissue that falls within the scoring region. For example, a Gleason score of prostate tissue is determined by classifying tissue patterns in the scoring region. Alternatively, a Gleason pattern is assigned by counting the number of third objects that possess a predetermined form.
7 Citations
25 Claims
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1. A method comprising:
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generating first objects from a first digital image of a first tissue slice, wherein the first tissue slice has been stained with a first stain that stains basal epithelial cells; generating second objects from a second digital image of a second tissue slice, wherein the second tissue slice has been stained with a second stain that stains luminal epithelial cells; co-registering the first digital image with the second digital image; defining third objects that include only those second objects that have more than a minimum separation from any of the first objects; defining a scoring region that includes the third objects; and determining a histopathological score in the scoring region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method comprising:
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segmenting a first digital image to generate first objects corresponding to tissue stained with a first stain, wherein the first stain stains basal membranes of the tissue; segmenting a second digital image to generate second objects corresponding to tissue stained with a second stain, wherein the second stain stains epithelial cells in the tissue; co-registering the first digital image and the second digital image; and displaying the second digital image on a graphical user interface with a subset of the second objects identified, wherein the subset of the second objects includes only those second objects that have at least a minimum separation from any of the first objects. - View Dependent Claims (17, 18, 19, 20, 21)
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22. A method comprising:
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identifying first objects in a digital image of a tissue slice, wherein the tissue slice has been stained with a first stain that stains basal epithelial cells and with a second stain that stains luminal epithelial cells; identifying second objects in the digital image of the tissue slice; defining third objects that include only those second objects that have more than a minimum separation from any of the first objects; counting a number of the third objects that possess a predetermined form; and determining a histopathological score based in part on the number of the third objects that possess the predetermined form. - View Dependent Claims (23, 24, 25)
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Specification