Method Of Using A Biosensor

• US 20140174951A1
• Filed: 02/28/2014
• Published: 06/26/2014
• Est. Priority Date: 02/06/2004
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Bayer Healthcare LLC (Bayer AG)
• Inventors: Beer, Greg P., Wu, Huan-Ping, Yip, Kin-Fai
• Granted Patent: US 9,410,917 B2
• Time in Patent Office: Days
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.

Claims

1-11. -11. (canceled)

12. A method of use of a biosensor including a mixture of an enzyme, a mediator, and an oxidizable species as an internal reference, said method comprising the steps of:

applying a first voltage potential in a first period;

providing a set delay period;

applying a second voltage potential in a final period following said delay period; and

wherein said first voltage potential and said second voltage potential are selectively provided for oxidizing only said mediator or both said mediator and said internal reference.

13. A method as recited in claim 12 wherein the step of applying a first voltage potential in a first period includes the step of applying a selected high first voltage potential in the first period for oxidizing said mediator and said internal reference.

14. A method as recited in claim 12 wherein the step of applying a first voltage potential in a first period includes the step of applying a selected low first voltage potential in the first period for oxidizing only said mediator.

15. A method as recited in claim 12 wherein the step of applying a second voltage potential in a final period following said delay period includes the step of applying a selected second voltage potential for oxidizing said mediator and said internal reference.

16. A method as recited in claim 12 wherein the step of applying a second voltage potential in a final period following said delay period includes the step of applying a selected second voltage potential for oxidizing only said mediator.

17. A method as recited in claim 12 wherein the steps of applying said first voltage potential and applying said second voltage potential includes the steps of applying a selected voltage potential in a range between 100 mV and 400 mV.

18. A method as recited in claim 12 wherein the steps of applying said first voltage potential and applying said second voltage potential includes the steps of applying a selected first voltage potential in the first period for oxidizing both said mediator and said internal reference;

and applying a selected second voltage potential for oxidizing only said mediator.

19. A method as recited in claim 12 wherein the biosensor includes a mediator comprising one of 3-phenylimino-3H-phenothiazine and ruthenium hexaamine;

and wherein the internal reference comprises ferrocyanide; and

wherein the steps of applying said first voltage potential and applying said second voltage potential includes the steps of applying a selected first and second voltage potential for oxidizing only said mediator.

20. A method as recited in claim 12 wherein the steps of applying said first voltage potential and applying said second voltage potential includes the steps of applying a selected first and second voltage potential for oxidizing both said mediator and said internal reference;

wherein said internal reference effectively anchoring a calibration intercept within a narrow range and said internal reference effectively maintaining a calibration slope for the biosensor.