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Compositions and Methods for Modifying Cells

  • US 20140178879A1
  • Filed: 02/28/2014
  • Published: 06/26/2014
  • Est. Priority Date: 10/31/2000
  • Status: Active Grant
First Claim
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1. A method for detecting the replacement of an endogenous allele with a modified allele in a cell, the method comprising:

  • (a) exposing the DNA of the cell of with a first probe and a second probe, wherein the first probe binds within the endogenous allele but not within the modified allele, and the second probe binds within a reference gene but not within the endogenous allele and not within the modified allele, and wherein both probes generate a detectable signal upon binding, and wherein the reference gene is of known copy number;

    (b) detecting the signal from the binding of the first probe and the signal from the binding of the second probe; and

    (c) comparing the signal from the binding of the second probe to the signal from the binding of the first probe, and determining from the comparison a copy number of the endogenous allele.

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