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Methods for Detection of Nucleotide Modification

  • US 20140178881A1
  • Filed: 07/27/2012
  • Published: 06/26/2014
  • Est. Priority Date: 07/29/2011
  • Status: Active Grant
First Claim
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1. A method of identifying a modified cytosine residue in a sample nucleotide sequence comprising;

  • (i) providing a population of polynucleotides which comprise the sample nucleotide sequence,(ii) oxidising or reducing a first portion of said population,(iii) treating the oxidised or reduced first portion of said population and a second portion of said population with bisulfite,(iv) sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and;

    (v) identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence.

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