ANTIBODY CATEGORIZATION BASED ON BINDING CHARACTERISTICS

• US 20140178905A1
• Filed: 10/01/2013
• Published: 06/26/2014
• Est. Priority Date: 12/03/2001
• Status: Abandoned Application

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Gallo, Michael, Jia, Xiao-Chi, Joho, Keith, Kang, Jaspal S., WALKER, Wynn L.
• Application Number:
  US14/043,399
• Publication Number:
  US 20140178905A1
• Time in Patent Office: Days
• US Class Current: 435/7.92
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/6878 in eptitope analysis
• CPC Class Codes: G06F 17/16 Matrix or vector computation , e.g. matrix-matrix or matrix-vector multiplication, matrix factorization matrix transposition G06F7/78
• CPC Class Codes: G16B 40/00 ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• CPC Class Codes: G16B 40/30 Unsupervised data analysis

Abstract

Methods for categorizing antibodies based on their epitope binding characteristics are described. Methods and systems for determining the epitope recognition properties of different antibodies are provided. Also provided are data analysis processes for clustering antibodies on the basis of their epitope recognition properties and for identifying antibodies having distinct epitope binding characteristics.

Claims

1. An antibody competition assay method for determining antibodies that bind to an epitope on an antigen, comprising:

providing a set of antibodies that bind to an antigen;

labelling each antibody of said set to form a labelled reference antibody set such that each labelled reference antibody is distinguishable from every other labelled reference antibody in said labelled reference antibody set;

selecting a probe antibody from said set of antibodies that bind to the antigen;

contacting said probe antibody with said labelled reference antibody set in the presence of said antigen;

detecting said probe antibody in a complex comprising said antigen, one labelled reference antibody bound to said antigen, and said labelled probe antibody bound to said antigen;

identifying each said labelled reference antibody bound to said antigen in each said complex;

determining whether said probe antibody competes with any reference antibody in said labelled reference antibody set, wherein competition indicates that said probe antibody binds to the same epitope as another antibody in said set of antibodies that bind to an antigen.