COMPOSITIONS AND METHODS FOR SELECTION OF NUCLEIC ACIDS CONTAINING MODIFIED BASES
First Claim
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1. A method of generating a pool of sequencing templates enriched for a type of modified base, the method comprising:
- a) fragmenting a nucleic acid sample comprising the type of modified base, thereby generating a mixture comprising a first subset of nucleic acid fragments comprising the type of modified base and a second subset of nucleic acid fragments that do not comprise the type of modified base;
b) linking adapters to nucleic acid fragments in both the first subset and the second subset; and
c) retaining the nucleic acid fragments in the first subset from the mixture, thereby generating a pool of sequencing templates enriched for the type of modified base.
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Abstract
Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of the initial population enriched for a desired property, or lacking nucleic acids having an undesired property. The methods are particularly useful for analyzing populations having a high degree of complexity, e.g., chromosomal-derived DNA, whole genomic DNA, or mRNA populations. In addition, such methods allow for analysis of pooled samples.
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20 Claims
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1. A method of generating a pool of sequencing templates enriched for a type of modified base, the method comprising:
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a) fragmenting a nucleic acid sample comprising the type of modified base, thereby generating a mixture comprising a first subset of nucleic acid fragments comprising the type of modified base and a second subset of nucleic acid fragments that do not comprise the type of modified base; b) linking adapters to nucleic acid fragments in both the first subset and the second subset; and c) retaining the nucleic acid fragments in the first subset from the mixture, thereby generating a pool of sequencing templates enriched for the type of modified base. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification