POST-SYNTHESIS PROCESSING SYSTEM FOR SUPPORTED OLIGONUCLEOTIDES, AND METHOD

• US 20140179565A1
• Filed: 11/25/2013
• Published: 06/26/2014
• Est. Priority Date: 09/22/2004
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Vann, Charles
• Application Number:
  US14/089,733
• Publication Number:
  US 20140179565A1
• Time in Patent Office: Days
• US Class Current: 506/27
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00454 by chemical cleavage from the solid support
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00542 Alphanumeric characters
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00695 Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/14 Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

A system is provided that can include: a plurality of retainment regions, where each retainment region is adapted to retain a respective type of an oligonucleotide supported on a respective support; a mixture retainment region; a handling device; a control unit adapted to control the handling device; and a separating unit adapted to simultaneously separate different supported oligonucleotides from their respective supports. A method is provided that can include: pooling together a plurality of supported oligonucleotides to form a mixture; and simultaneously separating the oligonucleotides of the supported oligonucleotides in the mixture from their supports. A method for facilitating genetic analysis is also provided and can include: receiving from a user a request for one or more genetic analysis assays; formulating each assay; and providing to the user the one or more assay; wherein the formulating can include pooling together into a mixture retainment region a plurality of different supported oligonucleotides and simultaneously separating the different oligonucleotides from their respective supports.

Claims

1. A system comprising:

a plurality of retainment regions, each retainment region being adapted to retain a respective type of oligonucleotide supported on a respective support;

a mixture retainment region;

a handling device;

a control unit programmed to control the handling device to pool in the mixture retainment region different supported oligonucleotides from different ones of the retainment regions, to form a pool; and

a separating unit adapted to simultaneously separate different types of oligonucleotides supported on different respective supports, from their respective supports.

2. The system of claim 1, further comprising different supported oligonucleotides in different ones of the retainment regions.

3. The system of claim 1, wherein the different supported oligonucleotides comprise a plurality ofdifferent types of supported oligonucleotides.

4. The system of claim 3, wherein the different types of supported oligonucleotides are respectively disposed in different retainment regions.

5. The system of claim 3, wherein each of the different types of supported oligonucleotides is disposed in two or more different retainment regions.

6. The system of claim 1, wherein the plurality of retainment regions are arranged in an addressable array and the control unit is programmed to control the handling device to access a plurality of retainment regions of the addressable array.

7. The system of claim 6, wherein access to each retainment region is provided by movement of the handling device.

8. The system of claim 6, wherein the control unit is adapted to receive a plurality of addresses corresponding to two or more retainment regions of the addressable array, and transfer one or more supported oligonucleotides from two or more of the plurality of retainment regions, to the mixture retainment region.

9. The system of claim 1, further comprising a purification unit that is adapted to purify the pool.

10. The system of claim 9, further comprising a mixture of components disposed in the mixture retainment region.

11. The system of claim 10, wherein the mixture of components comprises a mixture of supported oligonucleotides.

12. The system of claim 10, wherein the mixture of components comprises a mixture of separated oligonucleotides.

13. The system of claim 1, wherein the handling device is adapted to individually handle a single particle having a diameter of from about 10 microns to about 2000 microns.

14-30. -30. (canceled)

31. A system comprising:

a plurality of retainment regions, each retainment region being adapted to retain a respective type of chemical chemically bonded to a respective support;

a mixture retainment region;

a handling device;

a control unit programmed to control the handling device to pool in the mixture retainment region different supported chemicals from different ones of the retainment regions, to form a pool; and

a separating unit adapted to simultaneously separate different types of chemicals chemically bonded to different respective supports, from their respective supports.

32. The system of claim 31, further comprising different types of chemicals chemically bonded to respective supports, in different ones of the retainment regions.

33. The system of claim 31, wherein the plurality of retainment regions are arranged in an addressable array and the control unit is programmed to control the handling device to access a plurality ofretainment regions of the addressable array.

34. The system of claim 33, wherein the control unit is adapted to receive a plurality of addresses corresponding to two or more retainment regions of the addressable array, and transfer one or more supported chemicals from two or more of the plurality of retainment regions, to the mixture retainment region.

35. A method comprising:

pooling together a plurality of supported oligonucleotides to form a mixture, each supported oligonucleotide comprising an oligonucleotide attached to a support; and

simultaneously separating the oligonucleotides of the supported oligonucleotides in the mixture from their supports to form a pool of separated oligonucleotides.

36. The method of claim 35, further comprising packaging the pool of separated oligonucleotides in a container.

41. The method of claim 35, wherein said pooling comprises transferring the plurality of supported oligonucleotides supported on their supports to the mixture from a plurality of different respective retainment regions.

37-40. -40. (canceled)

42-57. -57. (canceled)