PIPETTE

• US 20140186235A1
• Filed: 12/27/2013
• Published: 07/03/2014
• Est. Priority Date: 12/28/2012
• Status: Active Grant

• Current Assignee: Access Bio, Inc.
• Original Assignee: Access Bio, Inc.
• Inventors: Kwak, Joon Hyeok, Koo, Tae-Hee, Kim, Hyeonsuk, Choi, Young Ho
• Granted Patent: US 9,656,258 B2
• Time in Patent Office: Days
• US Class Current: 422/520
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Abstract

A pipette for delivering a measured volume of liquid having an elongated hollow tubular body (or capillary tube) (2) configured to draw liquid by capillary action, the hollow tubular body having a lower open end and an upper open end; and a chamber (3) structurally connected to the upper end of the tubular body (2), the chamber having an orifice (4) in the upper portion of the chamber.

Claims

1. A pipette (1) for delivering a measured volume of liquid comprising:

an elongated hollow tubular body (2) configured to draw liquid by capillary action, the hollow tubular body having a lower open end and an upper open end; and

a chamber (3) structurally connected to the upper end of the tubular body (2);

(i) the chamber comprising two quadrilateral shaped sidewalls slightly curved such that when the sidewalls are facing each other and connected together by all four corners of the quadrilateral shaped sidewalls, the chamber has quadrilateral shaped sidewalls and an oval shaped inner area;

wherein, there is an orifice in the upper portion of one sidewall of the chamber;

the chamber extends to a middle tube extension and continues to a bottom tube extension;

the bottom tube extension being integrally united to said capillary tube at the lower end of said bottom extension;

the orifice on the one sidewall of the chamber being of a diameter such that the orifice is coverable with a finger or fingertip of the user; and

the elongated hollow tubular body (or capillary tube) (2) having a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action;

or

(ii) the chamber comprising a upper bulb (3-a), a lower sample collecting bulb (3-c), and a tubular passage (or connection tube) (3-b) connecting the upper bulb and the lower bulb, wherein the upper bulb has an orifice on one sidewall of the bulb;

the upper bulb and lower bulb are formed by quadrilateral shaped sidewalk slightly curved such that when the sidewalls are facing each other and connected together by all four corners of the quadrilateral shaped sidewalls, the chamber has quadrilateral shaped sidewalls and an oval shaped inner area;

the lower bulb (3-c) extends to a middle tube extension (3-c-i) and continues to a bottom tube extension (3-c-ii);

the bottom tube extension (3-c-ii) being integrally united to the elongated hollow tubular body (or capillary tube) (2) at the lower end of the bottom tube extension (3-c-ii);

the orifice on the one sidewall of the upper bulb (3-a) being of a diameter such that the orifice is coverable with a finger or fingertip of the user; and

the elongated hollow tubular body (or capillary tube) (2) having a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action.

2. The pipette according to claim 1, wherein the chamber extends directly to the bottom tube extension.

3. The pipette according to claim 1, further comprising an orifice on the opposite sidewall of the chamber so that both sidewalls of the chamber have an orifice.

4. The pipette according to claim 1, wherein the chamber extends directly to the bottom tube extension and further comprising an orifice on the opposite sidewall of the chamber so that both sidewalls of the chamber have an orifice.

5. The pipette according to claim 1, wherein the diameter and the height of the capillary tube are such that a collection of 2 μ

L of a liquid is drawn by capillary action.

6. The pipette according to claim 1, wherein the diameter of the capillary tube is 0.1 cm and the height of the capillary tube is 1.6 cm such that a collection of 2 μ

L of a liquid is drawn by capillary action.

7. The pipette according to claim 1, wherein the chamber has a diameter of 0.7 cm and a height of 2.4 cm.

8. The pipette according to claim 1, wherein the inner diameter of the capillary tube is 0.04 cm and the outer diameter of the capillary tube is 0.1 cm and the height of the capillary tube is 1.6 cm such that a collection of 2 μ

L of a liquid is drawn by capillary action.

9. The pipette according to claim 1, wherein the capillary tube having a diameter and a height of a predetermined measurement such that a precise collection of from about 2 μ

L to about 20 μ

L of a liquid is drawn by capillary action.

10. A pipette for delivering a precisely measured volume of liquid comprising:

a capillary tube (2) configured to draw liquid by capillary action, the capillary tube having a lower open end and an upper open end; and

a chamber (3) being structurally connected to the upper end of the capillary tube;

the chamber having an orifice (4) in the upper portion of the chamber;

wherein,

(i) the chamber extends to a middle tube extension (3-c-i) and continues to a bottom tube extension (3-c-ii);

the bottom tube extension being integrally united to the capillary tube at the lower end of the bottom extension;

the orifice is on one sidewall of the chamber at a selected distance from the open end; and

the capillary tube has a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action;

or

(ii) the chamber extends to a bottom tube extension (3-c-ii);

the bottom tube extension being integrally united to the capillary tube at the lower end of the bottom extension;

the orifice is on one sidewall of the chamber at a selected distance from the open end; and

the capillary tube has a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action.

11. The pipette according to claim 10, wherein the chamber is round or globular shape.

12. A pipette for delivering a measured volume of liquid comprising:

a capillary tube (2) configured to draw liquid by capillary action, the capillary tube having a lower open end and an upper open end; and

a chamber (3) being structurally connected to the upper end of the capillary tube, the chamber comprising an upper bulb and a lower bulb connected by a connection tube, wherein the upper bulb comprises an orifice;

wherein,

(i) the chamber extends to a middle tube extension and continues to a bottom tube extension, the bottom tube extension being integrally united to the capillary tube at the lower end of the bottom extension;

the orifice is made through one sidewall of the upper bulb of the chamber; and

the capillary tube has a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action;

or

(ii) the chamber extends to a bottom tube extension, the bottom tube extension being integrally united to the capillary tube at the lower end of the bottom extension;

the orifice is made through one sidewall of the upper bulb of the chamber; and

the capillary tube has a diameter and a height of a predetermined measurement such that a precise collection of from about 0.1 μ

L to about 200 μ

L of a liquid is drawn by capillary action.

13. The pipette according to claim 12, wherein the upper bulb and the lower bulb are of a substantially round or globular shape.

14. The pipette according to claim 12, wherein the upper bulb and the lower bulb of the chamber each has a diameter of 0.7 cm and a height of 1.0 cm.

15. The pipette according to claim 12, wherein the connection tube has a diameter of 0.25 cm and a height of 0.4 cm.

16. The pipette according to claim 12, wherein the middle tube extension has a diameter of 0.3 cm and a height of 1.3 cm and the bottom tube extension has a diameter of about 0.2 cm and a height of about 0.4 cm.

17. The pipette according to claim 12, further comprising an orifice on the opposite sidewall of the chamber so that both sidewalls of the chamber have an orifice.