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ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS

  • US 20140199730A1
  • Filed: 02/04/2014
  • Published: 07/17/2014
  • Est. Priority Date: 03/07/2007
  • Status: Active Grant
First Claim
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1. A method of whole genome amplification comprising:

  • a. generating a first plurality of droplets comprising polynucleotide primers;

    b. generating a second plurality of droplets comprising polynucleotides to be amplified, wherein the polynucleotides are obtained from a whole genome;

    c. merging a first droplet of the first plurality of droplets with a second droplet of the second plurality of droplets to form a coalesced droplet; and

    d. performing a whole genome amplification reaction on the polynucleotides in the coalesced droplet to form amplicons.

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