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Abstract
The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof.
256 Citations
166 Claims
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1-86. -86. (canceled)
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132. A construct comprising:
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a) a nucleic acid sequence encoding at least one clustered-regularly-spaced-short-palindromic-repeat-associated (cas) protein; b) a nucleic acid sequence comprising at least one Clustered Regularly Spaced Short Palindromic Repeat (CRISPR) repeat or a full length complement thereof; and
,c) a nucleic acid sequence comprising at least one CRISPR spacer or a full length complement thereof. - View Dependent Claims (133, 134, 135, 136, 137, 138, 139, 140, 141, 142)
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- 143. A recombinant CRISPR-cas combination comprising at least one cas protein encoded by a cas gene and at least one CRISPR repeat in combination with at least one CRISPR spacer.
- 154. A method of modifying a nucleic acid of interest in the genomic DNA of a cell, the method comprising introducing into said cell at least one cas gene or protein and at least one CRISPR repeat in combination with at least one CRISPR spacer.
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163. A recombinant nucleic acid comprising at least 50% of the wild type nucleotide sequence of SEQ ID NO:
- 462, 474, 489, 494, 499, 505 or 518.
- View Dependent Claims (164)
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165. A recombinant nucleic acid comprising a nucleotide sequence of at least 75% sequence identity to SEQ ID NO:
- 462, 474, 489, 494, 499, 505 or 518.
- View Dependent Claims (166)
Specification