METHOD FOR MEASURING CONCENTRATION OF ANALYTEIN BLOOD SAMPLE, AND BIOSENSOR SYSTEM

• US 20140209482A1
• Filed: 03/31/2014
• Published: 07/31/2014
• Est. Priority Date: 11/28/2008
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Company Limited (PHC Holdings Corporation)
• Inventors: UCHIYAMA, Motonori
• Granted Patent: US 9,658,182 B2
• Time in Patent Office: Days
• US Class Current: 205/777.5
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

The method includes: acquiring data a related to a temperature of a blood sample on a sensor chip, based on a dimension of a current flowing in the blood sample by applying a first voltage to the pair of electrodes in contact with the blood sample, the first voltage being set so as to reduce an effect of hematocrit on a temperature measurement result; acquiring data b related to the concentration of an analyte in the blood sample, based on a dimension of a current flowing in the blood sample by applying a second voltage that is equal to or less than the first voltage, utilizing a reaction mediated by an oxidoreductase that uses the analyte in the blood sample as a substrate; and measuring a concentration that determines the analyte concentration in the blood sample based on the data a and the data b.

Claims

1. A method for measuring a concentration of an analyte in a blood sample using a sensor chip, the sensor chip including a capillary and a pair of electrodes, the method including:

acquiring data a related to a temperature of the blood sample, based on a dimension of a current flowing in the blood sample by applying a first voltage to the pair of electrodes in contact with the blood sample, the first voltage being set so as to reduce an effect of hematocrit on a temperature measurement result;

acquiring data b related to the concentration of the analyte, based on a dimension of a current flowing in the blood sample by applying a second voltage that is equal to or less than the first voltage, utilizing a reaction mediated by an oxidoreductase that uses the analyte in the blood sample as a substrate; and

measuring a concentration that determines the analyte concentration in the blood sample based on the data a and the data b.

2. The method according to claim 1, wherein

the first voltage is between 1.0V and 3.0V, and the second voltage is between 0.05V and 1.0V.

3. The method according to claim 1, further includes correcting the data b based on the data a.

4. The method according to claim 1, further includes:

calculating a concentration x of the analyte in the blood sample based on the data b; and

correcting the concentration x based on the data a.

5. The method according to claim 1, further includes:

calculating a temperature t of the blood sample based on the data a; and

correcting the data b based on the temperature t.

6. The method according to claim 1, further includes:

calculating a temperature t of the blood sample based on the data a;

calculating a concentration x of the analyte in the blood sample based on the data b; and

correcting the concentration x based on the temperature t.

7. The method according to claim 1, wherein

the data a is acquired before the data b is acquired.

8. The method according to claim 1, wherein

the data a related to the temperature of the blood sample contains a temperature, and

the data b related to the concentration of the analyte contains a glucose concentration.

9. The method according to claim 1, wherein

the data b related to the concentration of the analyte contains hematocrit,

the data a related to the temperature of the blood sample contains a temperature, and

the data b related to the concentration of the analyte further contains a glucose concentration.

10. The method according to claim 1, wherein

the data b related to the concentration of the analyte contains a concentration or an amount of a reducing substance,

the data a related to the temperature of the blood sample contains a temperature, and

the data b related to the concentration of the analyte further contains a glucose concentration.

11. The method according to claim 1, wherein

at least two features of the data contained in the data a and the data b are measured at the same time,

the data a related to the temperature of the blood sample contains a temperature, and

the data b related to the concentration of the analyte contains a glucose concentration.

12. The method according to claim 11, wherein

the data contained in the data a and the data contained in the data b are measured independently.

13. The method according to claim 1, wherein

the data contained in the data a and the data contained the data b are measured in order of temperature, glucose concentration, concentration or amount of a reducing substance, and hematocrit,

the data a related to the temperature of the blood sample contains a temperature, and

the data b related to the concentration of the analyte contains a glucose concentration.

14. The method according to claim 11, wherein

the data contained in the data a and the data contained in the data b are measured through independent electrodes.

15. A biosensor system that measures a concentration of an analyte in a blood sample, the biosensor system including a sensor chip and a measuring device,

the sensor chip having temperature electrodes and analysis electrodes, the temperature electrodes being used for measuring a temperature of the blood sample, the analysis electrodes being used for measuring a feature related to the concentration of the analyte in the blood sample,

the measuring device including a control circuit configured to apply a voltage to the temperature electrodes and the analysis electrodes of the sensor chip,

the biosensor system comprising:

a voltage application unit configured to apply a first voltage to the temperature electrodes in accordance with the control circuit, the first voltage being set so as to reduce an effect of hematocrit on a temperature measurement result;

a temperature measuring unit configured to acquire data a related to the temperature of the blood sample, based on a dimension of a current flowing through the temperature electrodes in contact with the blood sample by the first voltage applied by the voltage application unit;

an analyte measuring unit configured to acquire data b related to the concentration of the analyte, based on a dimension of a current flowing through the analysis electrodes by a second voltage applied by the voltage application unit, utilizing a reaction mediated by an oxidoreductase that uses the analyte in the blood sample as a substrate, the second voltage being equal to or less than the first voltage; and

a concentration determination unit configured to determine an analyte concentration in the blood sample based on the data a and the data b.

16. The biosensor system according to claim 15, wherein

the first voltage is between 1.0V and 3.0V, and the second voltage is between 0.05V and 1.0V.

17. The biosensor system according to claim 15, wherein

the analyte measuring unit acquires the data b related to the concentration of the analyte after the analyte measuring unit acquires the data a related to the temperature of the blood sample.

18. The biosensor system according to claim 15, wherein

the concentration determination unit includes:

a temperature measuring unit configured to acquire data c related to the temperature of the blood sample, based on the dimension of the current flowing through the temperature electrodes in contact with the blood sample, after acquisition of the data b;

a computing unit configured to acquire data d related to the temperature of the blood sample by calculating the data a and the data c; and

a calculating unit configured to calculate the concentration x of the analyte corrected in accordance with the temperature of the blood sample, based on the data d.

19. The biosensor system according to claim 15, wherein

the concentration determination unit includes:

a temperature calculating unit configured to calculate a temperature t of the blood sample based on the data a;

a concentration calculating unit configured to calculate a concentration x of the analyte in the blood sample based on the data b;

an environmental temperature measuring unit configured to measure an environmental temperature t1 in a periphery of the blood sample;

a comparison unit configured to compare a difference between the temperature t and the environmental temperature t1 with a temperature threshold Z; and

a correction unit configured to correct the concentration x based on the temperature t when the relation |t−

t1|≧

Z is satisfied, and correcting the concentration x based on the temperature t1 when the relation |t−

t1|<

Z is satisfied.