HAND-HELD WIRELESS PLATFORM AND OPTICS FOR MEASUREMENT OF DNA, RNA, MICRORNAS, AND OTHER MARKERS OF PATHOGENS, GENETIC DISEASES, AND CANCER
First Claim
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1. A device, comprising:
- a) a plurality of sample wells, at least one of the wells containing one or more light emitting molecules;
b) a plurality of Light Emitting Diodes (LEDs), each LED capable of emitting optical energy for activating the one or more of the light emitting molecules in an associated sample, wherein each of the LEDs has a vertical plane;
c) a plurality of optical fibers, each of the optical fibers having first and second ends, each of the first ends separately and operably linked to an associated sample well for capturing emitted optical energy from the light emitting molecule(s), the second end configured for emitting the captured optical energy;
wherein the first end is at an angle of greater than 1° and
less than 90°
from the vertical plane of the LED.
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Abstract
The present invention provides compositions for making and methods of using a hand-held nucleic acid amplification device, comprising a disposable biochip with a series of sample wells, each sample well having a novel optical arrangement that includes a light-emitting diode (LED) and a single light capturing element (e.g. a photodiode) for quickly measuring light emissions from biological samples such as nucleic acid amplification reactions. Such a device can utilize isothermal amplification for obtaining detectable yields of amplified nucleic acid product in short time periods, for example, within seconds.
31 Citations
20 Claims
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1. A device, comprising:
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a) a plurality of sample wells, at least one of the wells containing one or more light emitting molecules; b) a plurality of Light Emitting Diodes (LEDs), each LED capable of emitting optical energy for activating the one or more of the light emitting molecules in an associated sample, wherein each of the LEDs has a vertical plane; c) a plurality of optical fibers, each of the optical fibers having first and second ends, each of the first ends separately and operably linked to an associated sample well for capturing emitted optical energy from the light emitting molecule(s), the second end configured for emitting the captured optical energy; wherein the first end is at an angle of greater than 1° and
less than 90°
from the vertical plane of the LED. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A microfluidic biochip, comprising:
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a plurality of units, each unit comprising a sample well, each sample well connected to a first branch of a bifurcated input microchannel and to an airlock microchannel, wherein each airlock microchannel allows sample to enter a sample well but does not allow components in the sample well to leave the sample well; and wherein each sample well comprises walls that allow passage of light radiating from with the sample well. - View Dependent Claims (19, 20)
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Specification