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METHODS AND SYSTEMS FOR ASSESSING SAMPLE BEHAVIOR IN A FLOW CYTOMETER

  • US 20140216128A1
  • Filed: 01/31/2014
  • Published: 08/07/2014
  • Est. Priority Date: 02/01/2013
  • Status: Active Grant
First Claim
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1. A method for generating an entrainment factor for a flow cytometer sample, the method comprising:

  • flowing the sample comprising a series of particles through the flow cytometer wherein each particle is associated with a signal;

    detecting a series of events with a detection system in a first time interval from a stream formed from the flow cytometer;

    calculating an expected frequency of events in the first time interval based on a characterizing distribution;

    measuring an observed frequency of events within a second time interval, wherein the second time interval is within the first time interval; and

    generating the entrainment factor based at least in part on a ratio of the observed frequency to the expected frequency.

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