Microbe Detection Via Hybridizing Magnetic Relaxation Nanosensors
First Claim
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1. A method of detecting a nucleic acid analyte of interest in a sample, the method comprising:
- (a) subjecting nucleic acid from the sample with at least one hybridizing magnetic relaxation nanosensor (hMRS), wherein said hMRS is configured to specifically hybridize to the nucleic acid analyte of interest; and
(b) detecting a change in the magnetic resonance signal from a hMRS hybridized to a nucleic acid analyte of interest in the nucleic acid from the sample;
thereby detecting the presence of the nucleic acid analyte of interest in the sample.
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Abstract
Disclosed herein are methods and materials for facilitating the detection of nucleic acid analytes of interest. Specifically exemplified herein are methods for detecting mycobacterial microorganisms, namely Mycobacterium avium spp. paratuberculosis. Also disclosed is new hybridizing magnetic relaxation nanosensor (hMRS) particularly adapted to detect a target nucleic acid analyte of interest.
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Citations
21 Claims
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1. A method of detecting a nucleic acid analyte of interest in a sample, the method comprising:
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(a) subjecting nucleic acid from the sample with at least one hybridizing magnetic relaxation nanosensor (hMRS), wherein said hMRS is configured to specifically hybridize to the nucleic acid analyte of interest; and (b) detecting a change in the magnetic resonance signal from a hMRS hybridized to a nucleic acid analyte of interest in the nucleic acid from the sample;
thereby detecting the presence of the nucleic acid analyte of interest in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14)
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9. (canceled)
- 15. A hybridizing magnetic relaxation nanosensor (hMRS), the hMRS comprising a magnetic nanoparticle associated with a nucleic acid that is configured to specifically hybridize to a nucleic acid analyte of interest.
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21-37. -37. (canceled)
Specification