AFFINITY-BASED PARTITION ASSAY FOR DETECTION OF TARGET MOLECULES
First Claim
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1. A method of detecting a target molecule in a sample, the method comprising:
- incubating a sample in a mixture with at least a first probe linked to a first label and a second probe linked to a second label, wherein the first and second probes specifically bind the same target molecule, if present;
partitioning the mixture into two or more partitions; and
detecting the presence of the first label and the second label in at least one same partition, thereby detecting the target molecule in the sample.
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Abstract
The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.
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Citations
20 Claims
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1. A method of detecting a target molecule in a sample, the method comprising:
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incubating a sample in a mixture with at least a first probe linked to a first label and a second probe linked to a second label, wherein the first and second probes specifically bind the same target molecule, if present; partitioning the mixture into two or more partitions; and detecting the presence of the first label and the second label in at least one same partition, thereby detecting the target molecule in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of detecting a target molecule in a sample, wherein the target molecule is linked to, or is capable of generating, a detectable molecule, the method comprising,
incubating the sample in a mixture with a first probe linked to a first label, wherein the first probe specifically binds the target molecule, if present; -
partitioning the mixture into two or more partitions; detecting the presence of the first label and the detectable molecule in at least one same partition, thereby detecting the target molecule in the sample. - View Dependent Claims (14)
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15. A method of detecting interaction of at least a first target molecule and a second target molecule that form a complex in a sample, the method comprising:
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incubating the sample in a mixture with a first probe linked to a first label and a second probe linked to a second label, wherein the first probe specifically binds the first target molecule, if present, and the second probe specifically binds the second target molecule, if present; partitioning the mixture into two or more partitions; and detecting the presence of the first label and the second label in at least one same partition, thereby detecting the target molecule in the sample. - View Dependent Claims (16)
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17. A method of detecting a target molecule or interaction of target molecules in a sample, the method comprising:
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incubating a sample in a mixture with at least a first probe linked to a first label that generates a detectable signal and a second probe linked to a second label, wherein the first probe specifically binds to the target molecule, if present in the sample, and wherein the second probe specifically binds to the same target molecule as the first probe or to a second target molecule that interacts with the target molecule that the first probe specifically binds to, if present in the sample; partitioning the mixture into two or more partitions; and detecting the quenching of the detectable signal generated by the first label and/or the second label in at least one partition;
thereby detecting the target molecule or interaction of target molecules in the sample. - View Dependent Claims (18)
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19. A method of detecting a target molecule in a sample, the method comprising:
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incubating a sample in a mixture with at least a first probe linked to a first label and a second probe linked to a second label, wherein the first probe specifically binds to a molecular component that interacts with the target molecule and the second probe competes with the target molecule for interacting with the molecular component; partitioning the mixture into two or more partitions; and detecting a reduction in the number of partitions expressing both the first label and the second label;
thereby detecting the target molecule.
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20. A partition library comprising two or more partitions, wherein at least some partitions comprise a first probe comprising a first label and a second probe comprising a second label.
Specification