ARTIFICIAL LEAF-LIKE MICROPHOTOBIOREACTOR AND METHODS FOR MAKING THE SAME

• US 20140242676A1
• Filed: 01/31/2014
• Published: 08/28/2014
• Est. Priority Date: 02/01/2013
• Status: Abandoned Application

• Current Assignee: Los Alamos National Security LLC (Government of the United States of America)
• Original Assignee: Los Alamos National Security LLC (Government of the United States of America)
• Inventors: Abdel-Fattah, Amr I., Hollingsworth, Jennifer Ann, He, Peng, Sayre, Richard Thomas, Fisher, Suzanne Zoe
• Application Number:
  US14/170,400
• Publication Number:
  US 20140242676A1
• Time in Patent Office: Days
• US Class Current: 435/257.1
• CPC Class Codes: C12M 25/01 Drops
• CPC Class Codes: C12M 25/16 Particles; Beads; Granular material; Encapsulation chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Described herein are algae carbon capture systems and biomass production systems, and more specifically, algal based microphotobioreactors (μPBRs) comprising a biocompatible polymer (e.g., hydrogel) containing algae, inorganic carbon, light-frequency shifting agents (e.g., quantum dots and/or dyes of fluorescent proteins) and methods for making such μPBRs.

Claims

1. A composition comprising a biocompatible polymer bead having inorganic carbon and algae.

2. The composition of claim 1, wherein the biocompatible polymer is a homopolymer or heteropolymer or combination thereof.

3. The composition of claim 1, wherein the biocompatible polymer comprises a polysaccharide.

4. The composition of claim 1, wherein the biocompatible polymer is a hydrogel foam.

5. The composition of claim 1, wherein the biocompatible polymer comprises cross-linked monomers selected from the group consisting or organic monomers, inorganic monomers and combinations thereof.

6. The composition of claim 1, wherein the biocompatible polymer comprises cross-linked monomers selected from the group consisting of alginate, agar, carrageenins, cellulose, a combination of silicone and/or siloxanes with polyacrlymide and combinations thereof.

7. The composition of claim 1, wherein the monomers of the biocompatible polymer are cross-linked with a multivalent cation.

8. The composition of claim 6, wherein the multivalent cation is selected from the group consisting of a metal cation, an amine, an amino acid derivative, a water-miscible organic solvent and combinations thereof.

9. The composition of claim 8, wherein the metal cation is selected from the group consisting of calcium, magnesium, iron, copper, zinc, mangenses, potassium, sodium, ammonia, biocompatible Lewis acid metals and combinations thereof.

10. The composition of claim 1, wherein the monomers of the biocompatible polymer are cross-linked with an anion.

11. The composition of claim 10, wherein the anion is selected from the group consisting of phosphate, selenate, nitrate, chloride sulfate and combinations thereof.

12. The composition of claim 1, wherein the volume of inorganic carbon in the biocompatible polymer is up to 60%.

13. The composition of claim 1, wherein the inorganic carbon is selected from the group consisting of carbon dioxide, carbonic acid, bicarbonate anion, carbonate and a combination thereof.

14. The composition of claim 1, wherein the inorganic carbon forms pockets in the biocompatible polymer having an average diameter of from 0.5 nm to about 10 nm.

15. The composition of claim 1, wherein the algae are modified to have increased light utilization efficiency compared to wild-type algae of the same strain.

16. The composition of claim 1, wherein the algae have a photosynthetic rate that is higher than wild-type algae of the same strain at saturating light.

17. The composition of claim 1, wherein the algae have at least 10% greater biomass than wild-type algae of the same strain.

18. The composition of claim 1, wherein the peripheral light harvesting antenna size of photosystem II of the algae is smaller than the peripheral light harvesting antenna size of photosystem II of wild-type algae of the same strain.

19. The composition of claim 1, wherein the ratio of chlorophyll a to chlorophyll b of green algae (Chlorophyta) is greater than the ratio of chlorophyll a to chlorophyll b of wild-type algae of the same strain.

20. The composition of claim 1, wherein the ratio of chlorophyll a to chlorophyll b of the algae is from about 3 to about 7.

21. The composition of claim 1, wherein the chlorophyll b content of the algae is reduced by an RNAi mechanism.

22. The composition of claim 1, wherein the algae comprise a siRNA that targets the chlorophyllide an oxygenase (CAO) gene.

23. The composition of claim 1, wherein the algae'"'"'s endogenous CAO gene levels are reduced compared to the CAO gene levels of a wild-type algae of the same strain.

24. The composition of claim 23, wherein the translation activity of the CAO gene is reduced or inhibited with a nucleic acid binding protein 1 (NAB1).

25. The composition of claim 1, wherein the algae is a transgenic algae expressing a protein comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:

1, 2, 3 and combination thereof.

26. The composition of claim 1, wherein the strain of algae is selected from the group consisting of Chlamydomonas reinhardtii, Chlorella sp., Synechocystis sp., Synechococcus, Anabaena sp., Cyclotella, Phaeodactylum sp., Crypthicodineum sp., Schizochytridum sp., Haematococcus sp., Arthrospira (Spirulina) sp, Dunaliella sp. and combination thereof.

27. The composition of claim 1, wherein the biocompatible polymer further comprises a light frequency-shifting agent.

28. The composition of claim 27, wherein the light frequency-shifting agent is red light emitting.

29. The composition of claim 27, wherein the light frequency-shifting agent absorbs light comprising the light spectrum of from ultraviolet to green light and emits light comprising red light.

30. The composition of claim 27, wherein the light frequency-shifting agent is selected from the group consisting of a quantum dot, a fluorescent protein and a combination thereof.

31. The composition of claim 27, wherein the association between the light frequency-shifting agent and the biocompatible polymer is selected from the group consisting of a covalent bond, non-bonded interactions and a combination thereof.

32. The composition of claim 30, wherein the light frequency-shifting agent is a colloidal nanocrystal quantum dot.

33. The composition of claim 32, wherein the colloidal nanocrystal quantum dot comprises an inner core having an average diameter of at least 1.5 nm and an outer shell, wherein the outer shell comprises multiple monolayers of an inorganic material.

34. The composition of claim 32, wherein the colloidal nanocrystal quantum dot outer shell comprises at least four monolayers of inorganic material.

35. The composition of claim 32, wherein the colloidal nanocrystal quantum dot outer shell comprises from about four to twenty monolayers of inorganic material.

36. The composition of claim 32, wherein the colloidal nanocrystal quantum dot exhibits an effective Stokes shift of at least 75 nm.

37. The composition of claim 32, wherein the colloidal nanocrystal quantum dot inner core comprises material selected from the group consisting of CuInS2, Zn3P2, GaP, GaAs, GaSb, InP, InAs, InSb, ZnS, ZnSe, ZnTe, CdSe, CdS, CdTe, PbS, PbSe, PbTe, and combinations thereof.

38. The composition of claim 32, wherein the colloidal nanocrystal quantum dot outer shell comprises material selected from the group consisting of ZnS, ZnSe, ZnTe, CdS, CdSe, CuGaS2, GaP, Cu20, AlP, AlAs, GaS, SnS2 and combinations thereof.

39. The composition of claim 32, wherein the colloidal nanocrystal quantum dot inner core and outer shell comprise, respectively, CuInS2 and ZnS, or CuInS2 and ZnSe, or InP and ZnS, or InP and ZnSe, or Zn3P2 and ZnS.

40. The composition of claim 30, wherein the light frequency-shifting agent is a fluorescent protein.

41. The composition of claim 30, wherein the fluorescent protein absorbs light comprising blue light and emits light comprising red light.

42. The composition of claim 30, wherein the fluorescent protein is a fusion protein of a green fluorescent protein (GFP) and a red fluorescent protein (RFP), wherein the fusion protein absorbs light comprising blue light and emits light comprising red light.

43. The composition of claim 1, wherein the biocompatible polymer further comprises an exogenous agent that is capable of converting carbon dioxide to bicarbonate.

44. The composition of claim 43, wherein the association between the exogenous agent and the biocompatible polymer is selected from the group consisting of a covalent bond, non-bonded interactions and a combination thereof

45. The composition of claim 43, wherein the exogenous agent is a carbonic anhydrase enzyme.

46. The composition of claim 45, wherein the amino acid sequence of the carbonic anhydrase enzyme is selected from the group consisting of SEQ ID NOs:

1, 2, 3 and a combination thereof.