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Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

  • US 20140242693A1
  • Filed: 12/30/2013
  • Published: 08/28/2014
  • Est. Priority Date: 12/31/2012
  • Status: Active Grant
First Claim
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1. A method of producing a three-dimensional cell cluster comprising the steps of:

  • a. growing pluripotent stem cells in a planar adherent culture,b. expanding the pluripotent stem cells to aggregated cell clusters, andc. transferring the clusters of pluripotent stem cells from the planar adherent culture to a dynamic suspension culture using an enzyme or chelating agent.wherein a three-dimensional cell cluster is formed.

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