ANTIBODIES COMPRISING CHIMERIC CONSTANT DOMAINS

• US 20140243504A1
• Filed: 01/31/2014
• Published: 08/28/2014
• Est. Priority Date: 02/01/2013
• Status: Active Grant

• Current Assignee: Regeneron Pharmaceuticals Incorporated
• Original Assignee: Regeneron Pharmaceuticals Incorporated
• Inventors: DAVIS, Samuel, SMITH, Eric, PATEL, Supriya
• Granted Patent: US 9,359,437 B2
• Time in Patent Office: Days
• US Class Current: 530/387.3
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 16/2809 against the T-cell receptor (TcR)-CD3 complex
• CPC Class Codes: C07K 16/2887 against CD20
• CPC Class Codes: C07K 16/2896 against molecules with a "CD"-designation, not provided for elsewhere
• CPC Class Codes: C07K 16/46 Hybrid immunoglobulins hybrids of an immunoglobulin with a peptide not being an immunoglobulin C07K19/00
• CPC Class Codes: C07K 2317/21 from primates, e.g. man
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/31 multispecific
• CPC Class Codes: C07K 2317/52 Constant or Fc region; Isotype
• CPC Class Codes: C07K 2317/53 Hinge
• CPC Class Codes: C07K 2317/71 Decreased effector function due to an Fc-modification
• CPC Class Codes: C07K 2317/732 Antibody-dependent cellular cytotoxicity [ADCC]
• CPC Class Codes: C07K 2317/734 Complement-dependent cytotoxicity [CDC]
• CPC Class Codes: C07K 2317/92 Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
• CPC Class Codes: C07K 2317/94 Stability, e.g. half-life, pH, temperature or enzyme-resistance
• CPC Class Codes: C12N 15/85 for animal cells

Abstract

Antibodies, antigen-binding proteins and Fc-fusion proteins that comprise recombinant polypeptides containing a chimeric heavy chain constant region sequence are provided that bind to certain Fc receptors however have reduced effector functions. Methods of making constructs for expression of such chimeric Fc-containing antibodies, antigen-binding proteins and Fc-fusion proteins in cell systems, and methods of producing and isolating the chimeric Fc-containing proteins are provided.

Claims

1. A recombinant polypeptide comprising a heavy chain constant (CH) region comprising, from N-terminus to C-terminus, a CH1 domain, a chimeric hinge, a CH2 domain, and a CH3 domain wherein:

(a) the CH1 domain comprises the amino acid sequence DKKV or DKRV from positions 212 to 215 (EU numbering),

(b) the chimeric hinge comprises a human IgG1 or a human IgG4 upper hinge amino acid sequence from positions 216 to 227 (EU numbering) and a human IgG2 lower hinge amino acid sequence PCPAPPVA (SEQ ID NO;

     3) from positions 228 to 236 (EU numbering),

(c) the CH2 domain comprises a human IgG4 CH2 domain amino acid sequence from positions 237 to 340 (EU numbering), and

(d) the CH3 domain comprises a human IgG1 or a human IgG4 CH3 domain sequence from positions 341 to 447 (EU numbering).

2. The recombinant polypeptide of claim 1, wherein the CH region has an amino acid sequence at least 99% identical to any one of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

30, SEQ ID NO;

31, SEQ ID NO;

37, or SEQ ID NO;

38.

3. The recombinant polypeptide of claim 1, wherein the CH1 domain comprises the amino acid sequence DKKV (SEQ ID NO:

4), and the chimeric hinge comprises the amino acid sequence EPKSCDKTHTCPPCPAPPVA (SEQ ID NO;

     8).

4. The recombinant polypeptide of claim 1, wherein the CH1 domain comprises the amino acid sequence DKRV (SEQ ID NO:

5), and the chimeric hinge comprises the amino acid sequence ESKYGPPCPPCPAPPVA (SEQ ID NO;

     9).

5. The recombinant polypeptide of claim 1, wherein the CH2 domain comprises the amino acid sequence SEQ ID NO:

10.

6. The recombinant polypeptide of claim 1, wherein the CH3 domain comprises the amino acid sequence selected from the group consisting of SEQ ID NO:

11, or SEQ ID NO;

12, SEQ ID NO;

41, and SEQ ID NO;

42.

7. The recombinant polypeptide of claim 1, wherein the recombinant polypeptide is an antigen-binding protein.

8. The recombinant polypeptide of claim 1, wherein the recombinant polypeptide is a receptor-Fc fusion protein.

9. The recombinant polypeptide of claim 1, wherein the recombinant polypeptide is an antibody.

10. The recombinant polypeptide of claim 9, wherein the antibody exhibits direct cytotoxic activity of less than 20% cytolysis, or less than 10%, or 5%, 4%, 3%, 2%, or even 0% or undetectable cytolysis, at an antibody concentration of at least 10 nM.

11. The recombinant polypeptide of claim 10, wherein the direct cytotoxic activity is at least about 5-fold less, or at least about 10-fold less than the direct cytotoxic activity of a corresponding antibody comprising a wild-type IgG1 or wild-type IgG4 CH region.

23. A composition comprising a recombinant polypeptide of claim 1 or claim 12.

24. A nucleic acid molecule encoding a recombinant polypeptide of claim 1 or claim 12.

25. The nucleic acid molecule of claim 24, wherein the recombinant polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

30, and SEQ ID NO;

31, SEQ ID NO;

37, and SEQ ID NO;

38.

26. The nucleic acid molecule of claim 24 having a nucleotide sequence greater than 99% sequence identity to the nucleotide sequence of SEQ ID NO:

28, SEQ ID NO;

29, SEQ ID NO;

32, SEQ ID NO;

33, SEQ ID NO;

35, or SEQ ID NO;

36.

27. The nucleic acid molecule of claim 25 comprising a nucleotide sequence selected the group consisting of SEQ ID NO:

28, SEQ ID NO;

29, SEQ ID NO;

32, and SEQ ID NO;

33, SEQ ID NO;

35, and SEQ ID NO;

36.

28. A vector comprising a nucleic acid molecule claim 25.

29. The vector of claim 28, wherein the nucleic acid molecule is operatively linked to an expression control sequence suitable for expression in a host cell.

30. The vector of claim 29, wherein the expression control sequence comprises a promoter selected from the group consisting of SV40, CMV, CMV-IE, CMV-MIE, UbC, RSV, SL3-3, MMTV, Ubi and HIV LTR.

31. The vector of claim 29, wherein the expression control sequence comprises an expression cassette selected from the group consisting of TetR-ER_LBDT2 fusion gene driven by the CMV-MIE promoter, blasticidin resistance gene driven by the SV40 promoter, and Arc-ER_LBDT2 fusion gene driven by the CMV-MIE promoter.

32. The vector of claim 30, wherein the promoter is a CMV-MIE/TetO or CMV-MIE/Arc hybrid promoter.

33. The vector of claim 30, comprising one or more selectable marker genes selected from the group consisting of bla, bls, BSD, bsr, Sh ble, hpt, tetR, tetM, npt, kanR and pac.

34. A cell comprising a nucleic acid of claim 24.

35. A cell comprising the vector of claim 28.

36. The cell of claim 34, wherein the nucleic acid is integrated into the genome of the cell.

37. The cell of claim 36 comprising a nucleic acid encoding a protein expression enhancer.

38. The cell of claim 37 comprising a nucleic acid encoding an XBP polypeptide.

39. The cell of claim 34, wherein the cell is a eukaryotic cell.

40. The cell of claim 34, wherein the cell is an animal cell.

41. The cell of claim 34, wherein the cell is a mammalian cell.

42. The cell claim 34, wherein the cell is a CHO cell.

43. The cell of claim 34, wherein the cell is a CHO-K1 cell.

12. A recombinant polypeptide, wherein the polypeptide comprises N′

-VD1-X1-Y1-Y2-X2-X3-C′

, wherein;

(a) N′

is the N-terminus and C′

is the C-terminus of the polypeptide,

(b) VD1 is an amino acid sequence comprising an antigen-binding domain,

(c) X1 is an amino acid sequence comprising a domain selected from the group consisting of an IgG1 CH1 domain, an IgG4 CH1 domain, and at least positions 212-215 (EU numbering) of an IgG1 or IgG4 CH1 domain,

(d) Y1 comprises an amino acid sequence from positions 216-227 (EU numbering) of an IgG1 or IgG4 hinge region,

(e) Y2 comprises the human IgG2 lower hinge region amino acid sequence PCPAPPVA (SEQ ID NO;

     3) from positions 228 to 236 (EU numbering),

(f) X2 is an amino acid sequence comprising an IgG4 CH2 domain, and

(g) X3 is an amino acid sequence comprising an IgG1 CH3 domain or an IgG4 CH3 domain.

13. The recombinant polypeptide of claim 12, wherein the polypeptide comprises an amino acid sequence at least 99% identical to the amino acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

30, SEQ ID NO;

31, SEQ ID NO;

37, or SEQ ID NO;

38.

14. The recombinant polypeptide of claim 12, wherein X1 comprises the amino acid sequence DKKV (SEQ ID NO:

4), and Y1-Y2 comprises a chimeric hinge consisting of the amino acid sequence EPKSCDKTHTCPPCPAPPVA (SEQ ID NO;

     8).

15. The recombinant polypeptide of claim 12, wherein X1 comprises the amino acid sequence DKRV (SEQ ID NO:

5), and Y1-Y2 comprises a chimeric hinge consisting of the amino acid sequence ESKYGPPCPPCPAPPVA (SEQ ID NO;

     9).

16. The recombinant polypeptide of claim 12, wherein X2 comprises the amino acid sequence of SEQ ID NO:

10.

17. The recombinant polypeptide of claim 12, wherein X3 comprises the amino acid sequence selected from the group consisting of SEQ ID NO:

11, SEQ ID NO;

12, SEQ ID NO;

41, and SEQ ID NO;

42.

18. The recombinant polypeptide of claim 12, wherein the recombinant polypeptide is an isolated antigen-binding protein.

19. The recombinant polypeptide of claim 12, wherein the recombinant polypeptide is a receptor-Fc fusion protein.

20. The recombinant polypeptide of claim 12, wherein the recombinant polypeptide is an isolated antibody.

21. The recombinant polypeptide of claim 20, wherein the antibody exhibits direct cytotoxic activity of less than 20% cytolysis, or less than 10%, or 5%, 4%, 3%, 2%, 2%, or even 0% or undetectable cytolysis, at an antibody concentration of at least 10 nM.

22. The recombinant polypeptide of claim 21, wherein the direct cytotoxic activity is at least about 5-fold less, or at least about 10-fold less than the direct cytotoxic activity of a corresponding antibody comprising a wild-type IgG1 or wild-type IgG4 CH region.