SUBSTANCE DETERMINATION METHOD
First Claim
1. A substance determination method for quantification of a substance to be determined that is contained in a specimen, the method comprising:
- using a biosensor comprising;
a cavity into which said specimen is supplied;
an electrode system including a working electrode and a counter electrode; and
a reaction layer containing an enzyme reacting specifically with the substance to be determined,wherein a determination potential, referenced to said counter electrode, is applied to said working electrode, anda reducing substance is generated through reaction between said substance to be determined and said reaction layer;
measuring an oxidation current resulting from oxidation of the reducing substance; and
applying to said working electrode at least once, a pulse of conditioning potential higher than said determination potential, with said counter electrode being defined as a reference, after said specimen is supplied into said cavity and before said determination potential is applied to said working electrode.
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Accused Products
Abstract
A technique allowing improved determination accuracy in quantifying a substance to be determined by lessening influence by a current component different from an oxidation current is provided. The oxidation current results from oxidation of a reducing substance generated through reaction between an enzyme and the substance to be determined. Current components are contained in a response current resulting from application of a determination potential, referenced to a counter electrode, to a working electrode. Since a conditioning potential higher than a determination potential is applied as a pulse to the working electrode, influence by a current component different from an oxidation current can be lessened. Thus, the response current can be measured in a stable manner and determination accuracy in quantification of a substance to be determined which is contained in a specimen can be improved.
3 Citations
20 Claims
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1. A substance determination method for quantification of a substance to be determined that is contained in a specimen, the method comprising:
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using a biosensor comprising; a cavity into which said specimen is supplied; an electrode system including a working electrode and a counter electrode; and a reaction layer containing an enzyme reacting specifically with the substance to be determined, wherein a determination potential, referenced to said counter electrode, is applied to said working electrode, and a reducing substance is generated through reaction between said substance to be determined and said reaction layer; measuring an oxidation current resulting from oxidation of the reducing substance; and applying to said working electrode at least once, a pulse of conditioning potential higher than said determination potential, with said counter electrode being defined as a reference, after said specimen is supplied into said cavity and before said determination potential is applied to said working electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A substance determination system for quantification of a substance to be determined that is contained in a specimen, the system comprising:
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a biosensor comprising; a cavity into which said specimen is supplied; an electrode system including a working electrode and a counter electrode; and a reaction layer containing an enzyme reacting specifically with the substance to be determined, wherein a reducing substance is generated through reaction between said substance to be determined and said reaction layer; and a determination instrument operatively connected to said biosensor and configured to; apply to said working electrode at least once, a pulse of conditioning potential higher than a determination potential, with said counter electrode being defined as a reference, after said specimen is supplied into said cavity and before the determination potential is applied to said working electrode, and to apply the determination potential, referenced to said counter electrode, to said working electrode, and to measure an oxidation current resulting from oxidation of the reducing substance. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification