CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes

• US 20140248702A1
• Filed: 04/22/2014
• Published: 09/04/2014
• Est. Priority Date: 12/12/2012
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation), Massachusetts Institute of Technology, The Broad Institute, Inc.
• Original Assignee: Massachusetts Institute of Technology, The Broad Institute, Inc.
• Inventors: ZHANG, Feng, CONG, Le
• Granted Patent: US 8,932,814 B2
• Time in Patent Office: Days
• US Class Current: 435/462
• CPC Class Codes: C12N 15/1082 Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 15/63 Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
• CPC Class Codes: C12N 15/8213 Targeted insertion of genes into the plant genome by homologous recombination
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 15/907 in mammalian cells
• CPC Class Codes: C12N 2310/10 Type of nucleic acid
• CPC Class Codes: C12N 2310/20 involving clustered regularly interspaced short palindromic repeats [CRISPRs]
• CPC Class Codes: C12N 2310/3519 Fusion with another nucleic acid
• CPC Class Codes: C12N 2310/531 Stem-loop; Hairpin
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113

Abstract

The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

Claims

1. A method of altering expression of at least one gene product comprising introducing into a eukaryotic cell containing and expressing a DNA molecule having a target sequence and encoding the gene product an engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated (Cas) system comprising one or more vectors comprising:

a) a first regulatory element operable in a eukaryotic cell operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and

b) a second regulatory element operable in a eukaryotic cell operably linked to a nucleotide sequence encoding a Type-II Cas9 protein,

wherein components (a) and (b) are located on same or different vectors of the system,

wherein the CRISPR-Cas system comprises two or more nuclear localization signals (NLSs),

wherein the Cas9 protein comprises one or more mutations in a catalytic domain,

whereby the guide RNA targets the target sequence and the Cas9 protein is a nickase that cleaves only one strand of the DNA molecule, whereby expression of the at least one gene product is altered; and

, wherein the Cas9 protein and the guide RNA do not naturally occur together.

2. The method of claim 1, wherein the method further comprises the insertion of a homologous recombination (HR) template into the cleaved strand of the DNA molecule.

3. The method of claim 1, wherein the expression of two or more gene products is altered.

4. The method of claim 1, wherein the CRISPR-Cas system comprises a trans-activating cr (tracr) sequence.

5. The method of claim 1, wherein the Cas9 protein comprises a mutation selected from the group comprising D10A, E762A, H840A, N854A, N863A or D986A with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.

6. The method of claim 1, wherein the Cas9 protein is codon optimized for expression in the eukaryotic cell.

7. The method of claim 1, wherein the eukaryotic cell is a mammalian or human cell.

8. The method of claim 1, wherein the expression of one or more gene products is altered by genome editing.

9. The method of claim 1, wherein the expression of one or more gene products is increased.

10. The method of claim 1, wherein the expression of one or more gene products is decreased.

11. The method of claim 1, wherein the one or more vectors are viral vectors.

12. The method of claim 11, wherein the one or more viral vectors are selected from the group consisting of retroviral, lentiviral, adenoviral, adeno-associated and herpes simplex viral vectors.

13. A CRISPR-Cas system-mediated genome editing method comprising introducing into a eukaryotic cell containing a polynucleotide having a sequence wherein the polynucleotide sequence includes a target sequence, an engineered, non-naturally occurring CRISPR-Cas system comprising one or more vectors comprising:

a) a first regulatory element operable in a eukaryotic cell operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA that hybridizes with the target sequence, and

b) a second regulatory element operable in a eukaryotic cell operably linked to a nucleotide sequence encoding a Type-II Cas9 protein,

wherein components (a) and (b) are located on same or different vectors of the system,

wherein the Cas9 protein comprises one or more mutations in a catalytic domain whereby the Cas9 protein is a nickase that cleaves only one strand of the polynucleotide,

wherein the CRISPR-Cas system comprises two or more NLSs,

whereby the polynucleotide sequence is modified through the CRISPR-Cas system directing sequence-specific genome editing; and

, wherein the Cas9 protein and the guide RNA do not naturally occur together.

14. The method of claim 13, wherein the sequence-specific genome editing comprises high-fidelity homology-directed repair (HDR).

15. The method of claim 13, wherein the sequence-specific genome editing comprises creation of a nick in the polynucleotide which is repaired by a homologous recombination (HR) cell repair mechanism incorporating a HR template into the polynucleotide thereby modifying the polynucleotide sequence.

16. The method of claim 13, wherein the expression of two or more gene products is altered.

17. The method of claim 13, wherein the CRISPR-Cas system comprises a tracr sequence.

18. The method of claim 13, wherein the Cas9 protein comprises a mutation selected from the group comprising D10A, E762A, H840A, N854A, N863A or D986A with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.

19. The method of claim 13, wherein the Cas9 protein is codon optimized for expression in the eukaryotic cell.

20. The method of claim 13, wherein the eukaryotic cell is a mammalian or human cell.

21. The method of claim 13, wherein the expression of one or more gene products is increased.

22. The method of claim 13, wherein the expression of one or more gene products is decreased.

23. The method of claim 13, wherein the one or more vectors are viral vectors.

24. The method of claim 23, wherein the one or more viral vectors are selected from the group consisting of retroviral, lentiviral, adenoviral, adeno-associated and herpes simplex viral vectors.

25. An engineered, programmable, non-naturally occurring Type II CRISPR-Cas system comprising a Cas9 protein and at least one guide RNA that targets and hybridizes to a target sequence of a DNA molecule in a eukaryotic cell, wherein the DNA molecule encodes and the eukaryotic cell expresses at least one gene product, wherein the Cas9 protein comprises one or more mutations in a catalytic domain whereby the Cas9 protein is a nickase that cleaves only one strand of the DNA molecule, wherein the CRISPR-Cas system comprises two or more NLSs, whereby expression of the at least one gene product is altered;

and, wherein the Cas9 protein and the guide RNA do not naturally occur together.

26. The CRISPR-Cas system of claim 25, wherein the CRISPR-Cas system comprises a tracr sequence.

27. The method of claim 25, wherein the Cas9 protein comprises a mutation selected from the group comprising D10A, E762A, H840A, N854A, N863A or D986A with reference to the position numbering of a Streptococcus pyogenes Cas9 protein.

28. The method of claim 27, wherein the Cas9 protein comprises the mutation D10A.

29. The method of claim 27, wherein the Cas9 protein comprises the mutation H840A.

30. The CRISPR-Cas system of claim 25, wherein the eukaryotic cell is a mammalian or human cell.