Small Volume In Vitro Analyte Sensor
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
3 Citations
34 Claims
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1-18. -18. (canceled)
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19. A method for determining a concentration of an analyte in a sample, comprising:
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contacting a sample fluid with a sensor positioned in an analyte meter, wherein the sensor comprises; a first electrode, a second electrode, and a third electrode; a sample chamber for holding the sample fluid, the sample chamber comprising the first electrode, the second electrode, and the third electrode; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; applying a first potential between the first and second electrodes using the analyte meter and detecting a change in an electrical signal between the first and second electrodes to determine when the sample fluid contacts the first and second electrodes; applying a second potential between the first and third electrodes using the analyte meter and determining whether a change in an electrical signal occurs between the first and third electrodes to determine whether the sample fluid contacts the first and third electrodes; and determining an analyte concentration only upon detecting a change in an electrical signal between the first and third electrodes. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification