Small Volume In Vitro Analyte Sensor
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
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Citations
34 Claims
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1-18. -18. (canceled)
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19. A method of using an analyte meter, comprising:
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contacting a sample fluid with a sensor positioned in an analyte meter, wherein the sensor comprises; a first pair of electrodes; a second pair of electrodes; a sample chamber for holding the sample fluid, the sample chamber comprising the first pair of electrodes and the second pair of electrodes; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; applying a potential between the first pair of electrodes using the analyte meter and detecting a change in an electrical signal between the first pair of electrodes to determine when the sample fluid contacts the first pair of electrodes; applying a potential between the second pair of electrodes using the analyte meter and determining whether a change in an electrical signal occurs between the second pair of electrodes to determine whether the sample fluid contacts the second pair of electrodes; and preventing an analyte concentration determining assay from running when a change in an electrical signal does not occur between the second pair of electrodes. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification