METHOD FOR DETERMINING TEAR GLUCOSE CONCENTRATION WITH BLOOD GLUCOSE TEST STRIPS

• US 20140262830A1
• Filed: 03/13/2014
• Published: 09/18/2014
• Est. Priority Date: 03/13/2013
• Status: Abandoned Application

• Current Assignee: Eyelab Group, LLC, Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Cohan, Bruce E., Meyerhoff, Mark E., Cha, Kyoung Ha, Jensen, Gary C.
• Application Number:
  US14/208,891
• Publication Number:
  US 20140262830A1
• Time in Patent Office: Days
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

A method for determining glucose concentration in tear fluid includes providing a blood glucose test strip having glucose dehydrogenase as an active enzyme provided therein, receiving a tear fluid sample via fluid communication of the blood glucose test strip with an eye region of a subject, and processing the tear fluid sample to determine a tear glucose concentration. The method may further include correlating the determined tear glucose concentration with a blood glucose concentration.

Claims

1. A method for determining glucose concentration in tear fluid, the method comprising:

providing a blood glucose test strip having glucose dehydrogenase as an active enzyme provided therein;

receiving a tear fluid sample via fluid communication of the blood glucose test strip with an eye region of a subject; and

processing the tear fluid sample to determine a tear glucose concentration.

2. The method of claim 1, wherein receiving the tear fluid sample includes obtaining a tear fluid sample of less than about 1 μ

L.

3. The method of claim 1, wherein processing the tear fluid sample includes a processing time of about 5 seconds.

4. The method of claim 1, wherein the blood glucose test strip includes pyrroloquinoline quinone as a coenzyme.

5. The method of claim 1, wherein the blood glucose test strip includes a nitrosoaniline derivative as an electron transfer mediator.

6. The method of claim 1, wherein the blood glucose test strip includes electrodes provided therein, and wherein processing the tear fluid sample includes applying a voltage to the electrodes to induce an electrochemical reaction of the active enzyme and the glucose in the tear fluid sample, and detecting a current produced by the electrochemical reaction from which the tear glucose concentration is determined.

7. The method of claim 6, wherein the electrodes include gold electrodes.

8. The method of claim 6, wherein the voltage applied to the electrodes is between about 150 mV and 200 mV.

9. The method of claim 1, wherein processing the tear fluid sample includes detecting glucose concentrations between about 0-500 μ

M in the tear fluid sample.

10. The method of claim 1, wherein providing a blood glucose test strip includes selecting a blood glucose test strip from a single lot of test strips.

11. The method of claim 1, further comprising correlating the determined tear glucose concentration with a blood glucose concentration.

12. A method for determining glucose concentration in tear fluid, the method comprising:

providing a blood glucose test strip having glucose dehydrogenase as an active enzyme, pyrroloquinoline quinone as a coenzyme, and a nitrosoaniline derivative as an electron transfer mediator;

receiving a tear fluid sample via fluid communication of the blood glucose test strip with an eye region of a subject; and

processing the tear fluid sample to determine the glucose concentration in the tear fluid sample.

13. The method of claim 12, wherein receiving the tear fluid sample includes obtaining a tear fluid sample of less than about 1μ

L.

14. The method of claim 12, wherein processing the tear fluid sample includes a processing time of about 5 seconds.

15. The method of claim 12, wherein the blood glucose test strip includes gold electrodes provided therein, and wherein processing the tear fluid sample includes applying a voltage to the electrodes to induce an electrochemical reaction of the active enzyme, coenzyme and electron transfer mediator with the glucose in the tear fluid sample, and detecting a current produced by the electrochemical reaction from which the tear glucose concentration is determined.

16. The method of claim 15, wherein the voltage applied to the electrodes is about 150 mV.

17. The method of claim 12, wherein processing the tear fluid sample includes detecting glucose concentrations less than about 15 μ

M.

18. The method of claim 12, wherein providing a blood glucose test strip includes selecting a glucose test strip from a single lot of test strips.

19. The method of claim 12, further comprising correlating the determined tear glucose concentration with a blood glucose concentration.

20. A method for determining blood glucose concentration, the method comprising:

providing a blood glucose test strip having glucose dehydrogenase as an active enzyme provided therein;

receiving a tear fluid sample having a volume of less than about 1 μ

L via fluid communication of the blood glucose test strip with an eye region of a subject;

reacting the tear fluid sample with the active enzyme to determine the tear glucose concentration; and

correlating the determined tear glucose concentration with a blood glucose concentration.