Portable Blood Count Monitor

• US 20140270458A1
• Filed: 03/12/2014
• Published: 09/18/2014
• Est. Priority Date: 03/13/2013
• Status: Abandoned Application

• Current Assignee: TIRHR, LLC
• Original Assignee: Tahoe Institute For Rural Health Research, LLC, Regents of the University of California (University of California)
• Inventors: Hood, James, Tatsukawa, Keith, Smith, Zachary, Gao, Tingjuan, Lane, Stephen, Wachmann-Hogiu, Sebastian, Dwyre, Denis, Heifetz, Laurence, Matthews, Dennis
• Application Number:
  US14/207,300
• Publication Number:
  US 20140270458A1
• Time in Patent Office: Days
• US Class Current: 382/134
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972

Abstract

Devices, systems, and methods are disclosed for determining the number and type of blood cells in a blood sample. The blood sample is collected and held in a slide. In the slide, the blood sample is separated and channeled into at least two sampling chambers, one for red blood cells, another for white blood cells, and optionally yet another for platelets. The sampling chambers have wetting agents, lysing agents, staining agents, or the like therein to mix with the blood and facilitate cell count. The slide is placed in a portable slide analyzer where the sampling chambers are illuminated and images of the sampling chambers are taken. These images are converted into electronic form and sent by a communications module of the slide analyzer to a remote external location where the images are analyzed to determine the number and type of blood cells in the blood sample.

Claims

1. A system for determining the number and type of blood cells in a sample, the system comprising:

a slide for collecting and holding a blood sample of less than or equal to 5 uL, wherein the slide comprises a blood inlet and at least two sampling chambers; and

a slide analyzer for receiving and analyzing the slide, the slide analyzer comprising a light source configured to project light onto the slide, a slide receiver for receiving the slide, an optics assembly, an image receiver for capturing one or more images from the at least two sampling chambers, and an image analyzer for analyzing the captured images to determine the number and type of blood cells in the sample.

2. The system of claim 1, wherein the blood sample has a volume of less than or equal to 2 uL.

3. The system of claim 1, wherein the at least two chambers comprises at least one of a surfactant, a dying agent, a lysing agent, a dry form reagent, a liquid reagent, or a predetermined volume of a diluent.

4. The system of claim 1, wherein the slide further comprises at least one channel in fluid communication with the at least two sampling chambers.

5. The system of claim 4, wherein the slide further comprises a suction port in fluid communication with the at least one channel.

6. The system of claim 4, wherein the at least two sampling chambers comprises a first chamber for analyzing red blood cells and a second chamber for analyzing white blood cells.

7. The system of claim 6, wherein the at least one channel and the dimensions of the first and second chambers are configured so that a first predetermined volume of the blood sample enters into the first chamber and a second predetermined volume of the blood sample enters into the second chamber.

8. The system of claim 4, wherein the at least two sampling chambers further comprises a third chamber for analyzing platelets.

9. The system of claim 8, wherein the at least one channel and the dimensions of the first, second, and third chambers are configured so that a first predetermined volume of the blood sample enters into the first chamber, a second predetermined volume of the blood sample enters into the second chamber, and a third predetermined volume of the blood sample enters into the third chamber.

10. The system of claim 1, wherein the light source of the slide analyzer comprises an LED.

11. The system of claim 1, wherein the light source of the slide analyzer comprises a first light source and a second light source,

wherein the first light source, the received slide, and the optics assembly form a first optical path, and

wherein the second light source and the received slide form a second optical path at an angle to the first optical path.

12. The system of claim 1, wherein the optics assembly of the slide analyzer is configured to automatically focus on a bottom surface of the at least two sampling chambers.

13. The system of claim 1, wherein the optics assembly of the slide analyzer further comprises a light filter assembly.

14. The system of claim 1, wherein the optics assembly of the slide analyzer further comprises a magnifier.

15. The system of claim 1, wherein the image receiver of the slide analyzer comprises a CCD or CMOS detector array.

16. The system of claim 1, wherein the image analyzer of the slide analyzer comprises a processor adapted to analyze images taken by the image receiver to determine the number and type of blood cells present in the blood sample.

17. The system of claim 1, wherein the slide analyzer further comprises a memory module coupled to the image processor for storing images taken from the at least two sampling chambers through the optics assembly and the image receiver.

18. The system of claim 1, wherein the slide analyzer further comprises a communications module coupled to the image processor for communicating at least one of images taken from the at least two sampling chambers through the optics assembly and the image receiver and or analysis data thereof to an external source.

19. The system of claim 18, wherein the communications module communicates with the external source through at least one of telemetry, a satellite connection, a wireless connection, the Internet, e-mail, text messaging, or the like.

20. The system of claim 19, wherein the external source comprises a processor adapted to analyze the images communicated from the communications module of the slide receiver.

21. The system of claim 1, wherein the system is configured for use by a non-professionally trained user.

22. A method for determining the number and type of blood cells in a sample, the method comprising:

collecting a blood sample of less than or equal to 5 uL through an inlet of a slide;

channeling the blood sample into a first sampling chamber and a second sampling chamber;

receiving the slide in a slide analyzer;

acquiring an image of the first sampling chamber of the slide analyzer;

acquiring an image of the second sampling chamber of the slide analyzer;

analyzing the image of the first sampling chamber to determine at least one of the number and size of red blood cells in the blood sample; and

analyzing the image of the second sampling chamber to determine the number or type of white blood cells in the blood sample.

23. The method of claim 22, further comprising sending the image of the first sampling chamber and the image of the second sampling chamber with a communications module of the slide analyzer to an external location, wherein the images of the first and second sampling chambers are analyzed with a processor at the external location.

24. The method of claim 22, wherein the slide analyzer comprises a movable slide receiver for receiving the slide,

wherein acquiring the image of the first sampling chamber comprises moving the slide receiver so that the first sampling chamber is in optical alignment with an optics assembly of the slide assembly, and

wherein acquiring the image of the second sampling chamber comprises moving the slide receiver so that the second sampling chamber is in optical alignment with an optics assembly of the slide assembly.

25. The method of claim 22, wherein channeling the blood sample into a first chamber and a second chamber further comprises channeling the blood sample into a third chamber.

26. The method of claim 25, further comprising:

acquiring an image of the third sampling chamber; and

analyzing the image of the third sampling chamber to determine the number of platelets in the blood sample.

27. The method of claim 26, further comprising sending the image of the third sampling chamber to the external location with a communications module of the slide analyzer, wherein the image of the third sampling chamber is analyzed with a processor at the external location.

28. The method of claim 22, wherein the collected blood sample has a volume of less than or equal to 2 uL.

29. The method of claim 22, wherein a first predetermined volume of the blood sample is channeled into the first sampling chamber and a second predetermined volume of the blood sample is channeled into the second sampling chamber.

30. The method of claim 22, further comprising automatically focusing imaging optics of the slide analyzer to focus on bottom surfaces of the first sampling chamber and second sampling chamber before acquiring the images of the first sampling chamber and the second sampling chamber.

31. The method of claim 27, wherein the communications module communicates with the external source through at least one of telemetry, a satellite connection, a wireless connection, the Internet, e-mail, text messaging, or the like.

32. The method of claim 22, further comprising mixing at least one of a surfactant, a dying agent, a lysing agent, a dry form reagent, a liquid reagent, or a predetermined volume of a diluents present in the first or second chamber with the blood sample channeled into the first and second chambers.

33. The method of claim 32, further comprising lysing red blood cells in the second chamber with the lysing agent before analyzing the image of the second sampling chamber with the processor of the slide analyzer to determine the number or type of white blood cells in the blood sample.

34. The method of claim 33, wherein the lysing agent is selected from the group comprising SDS, saponins, snake venom, quaternary ammonium salts, triton-X, and the like.

35. The method of claim 32, wherein the dying agent comprising a nucleic acid staining agent, and wherein analyzing the image of the second sampling chamber with a processor of the slide analyzer to determine the number or type of white blood cells in the blood sample comprises:

staining the white blood cells in the second sampling chamber with the nucleic acid staining agent;

determining a relationship between fluorescence at a first color with fluorescence at a second color for individual white cells of the white blood cells in the sampling chamber; and

determining the type of the individual white blood cell based on the determined relationship.

36. The method of claim 35, wherein the nucleic acid staining agent comprises one or more of acridine orange, thiozole orange, acridine red, 7-AAD, LDS 751, and hydroxystilbamidine.

37. The method of claim 22, further comprising illuminating the first and second sampling chambers before or during the acquiring of the images of the first and second sampling chambers.

38. The method of claim 22, further comprising calibrating the slide analyzer before acquiring the images of the first and second sampling chambers.

39. The method of claim 38, wherein calibrating the slide analyzer comprises acquiring an image of a calibration chamber of the slide analyzer, analyzing the image of the calibration chamber to determine the number or type of cell reproductions in the calibration chamber, and comparing the determined number or type of cell reproductions with a predetermined number or type to determine the accuracy of the slide analyzer.