Portable Blood Count Monitor

• US 20140273064A1
• Filed: 03/12/2014
• Published: 09/18/2014
• Est. Priority Date: 03/13/2013
• Status: Active Grant

• Current Assignee: TIRHR, LLC
• Original Assignee: Tahoe Institute For Rural Health Research, LLC, Regents of the University of California (University of California)
• Inventors: Smith, Zachary, Gao, Tingjuan, Lane, Stephen, Wachmann-Hogiu, Sebastian, Dwyre, Denis, Heifetz, Laurence, Matthews, Dennis, Hood, James, Tatsukawa, Keith
• Granted Patent: US 10,024,858 B2
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: G01N 33/5017 for testing neoplastic activity
• CPC Class Codes: G01N 33/57438 of liver, pancreas or kidney

Abstract

This disclosure describes the development of a sample preparation, measurement, and analysis method that permits accurate characterization of red blood cells, platelets, and white blood cells, including a 3-part differential of the white blood cells count, to be performed on small volumes of a biological sample. This method is compatible with compact and portable instrumentation that permits the sample collection to be performed in a subject'"'"'s home and analysis to be performed elsewhere by transmission of the data to a laboratory or doctor'"'"'s office.

Claims

1. A device comprising:

a) a slide configured to receive a body fluid, wherein the slide comprises;

i) a first chamber, wherein the first chamber contains a first reagent capable of detecting a first analyte in the body fluid; and

ii) a second chamber, wherein the second chamber contains a second reagent capable of detecting a second analyte in the body fluid; and

b) an imaging system configured to acquire visual data from the slide.

2. The device of claim 1, further comprising a transmitter, wherein the transmitter is configured to transmit the acquired visual data to a receiver.

3. The device of claim 2, wherein the transmission is wireless.

4. The device of claim 1, wherein the body fluid is urine.

5. The device of claim 1, wherein the body fluid is blood.

6. The device of claim 1, wherein the first analyte is a red blood cell.

7. The device of claim 6, wherein the second analyte is a white blood cell.

8. The device of claim 1, wherein the slide and the imaging system are contained in a common housing.

9. The device of claim 8, wherein the slide is removable.

10. The device of claim 9, wherein the slide further comprises a channel across a surface of the slide, wherein the channel is in connection with the first chamber and the second chamber.

11. The device of claim 10, wherein the slide further comprises a port, wherein the port is configured to receive the body fluid and pass the body fluid through the channel to at least one chamber.

12. The device of claim 1, wherein the slide further comprises a third chamber, wherein the third chamber contains a third reagent capable of detecting a third analyte in the body fluid.

13. The device of claim 12, wherein the third analyte is a platelet.

14. The device of claim 1, wherein the slide further comprises at least one control chamber, wherein the control chamber contains a control analyte.

15. The device of claim 1, wherein the slide is holds no greater than 5 microliters of body fluid.

16. A device comprising:

a) a slide configured to receive a body fluid;

b) an imaging system configured to acquire visual data from the slide; and

c) a transmitter configured to wirelessly-transmit the acquired visual data over a distance of at least one mile.

17. The device of claim 16, wherein the device has a mass of no greater than 2,000 g.

18. The device of claim 16, wherein the imaging system acquires visual data from at least two different parts of the slide.

19. The device of claim 16, wherein the slide and the imaging system are contained in a common housing.

20. The device of claim 19, wherein the slide is removable.

21. The device of claim 16, wherein the body fluid is saliva.

22. The device of claim 16, wherein the body fluid is blood.

23. The device of claim 16, wherein the imaging system is configured to detect an analyte in the body fluid.

24. The device of claim 23, wherein the analyte is a red blood cell.

25. The device of claim 23, wherein the analyte is a white blood cell.

26. The device of claim 23, wherein the analyte is a platelet.

27. The device of claim 16, wherein the slide holds no greater than 5 microliters of body fluid.

28. A method for analyzing a body fluid, the method comprising:

a) providing the body fluid to a slide, wherein the slide comprises a first chamber and a second chamber;

b) detecting in the first chamber a first analyte in the body fluid with a first reagent;

c) detecting in the second chamber a second analyte in the body fluid with a second reagent;

d) acquiring by an imaging system visual data from the slide; and

e) transmitting the acquired visual data to a receiver by a transmitter.

29. The method of claim 28, wherein from about 1 microliter to about 5 microliters of body fluid is provided to the slide.

30. The method of claim 28, wherein the transmission is wireless.

31. The method of claim 30, wherein the transmitter and the receiver are at least one mile apart.

32. The method of claim 28, wherein the body fluid is saliva.

33. The method of claim 28, wherein the body fluid is blood.

34. The method of claim 28, wherein the first analyte is a red blood cell.

35. The method of claim 34, wherein the second analyte is a white blood cell.

36. The method of claim 35, wherein the slide further comprises a third chamber, wherein the method further comprises detecting in the third chamber a third analyte in the body fluid with a third reagent.

37. The method of claim 36, wherein the third analyte is a platelet.

38. The method of claim 28, wherein the slide further comprises a channel across the surface of the slide, wherein the channel is in connection with the first chamber and the second chamber.

39. The method of claim 28, wherein the device further comprises a port, wherein the port is configured to receive the body fluid and pass the body fluid through the channel to at least one chamber.

40. The method of claim 28, further comprising converting the acquired visual data to an image.

41. A system comprising:

a) a device comprising;

i) a slide configured to receive a body fluid;

ii) an imaging system configured to acquire visual data from the slide; and

iii) a transmitter, wherein the transmitter wirelessly transmits the acquired visual data; and

b) a receiver that receives the wirelessly-transmitted visual data from the transmitter, wherein the transmitter and the receiver are configured to communicate over a distance of at least 1 mile.

42. The system of claim 41, wherein the device has a mass of no greater than 2,000 g.

43. The system of claim 41, wherein the imaging system is configured to acquire visual data from at least two different parts of the slide.

44. The system of claim 41, wherein the slide, the imaging system, and the transmitter are contained in a common housing.

45. The system of claim 41, wherein the body fluid is saliva.

46. The system of claim 41, wherein the body fluid is blood.

47. The system of claim 41, wherein the slide comprises at least two chambers.

48. The system of claim 41, wherein the slide further comprises a channel across the surface of the slide, wherein the channel is in connection with a first chamber and a second chamber.

49. The system of claim 41, wherein the imaging system is configured to detect an analyte in the body fluid.

50. The system of claim 49, wherein the analyte is a red blood cell.

51. The system of claim 49, wherein the analyte is a white blood cell.

52. The system of claim 49, wherein the analyte is a platelet.

53. The system of claim 41, wherein the slide holds no greater than 5 microliters of body fluid.

54. The system of claim 41, wherein the receiver is in communication with a computer system configured to generate an image based on the acquired visual data.

55. A kit comprising:

a) a device comprising;

i) a slide configured to receive a body fluid, wherein the slide comprises a first chamber and a second chamber; and

ii) an imaging system configured to acquire visual data from the slide;

b) a first reagent capable of detecting a first cell type in the body fluid; and

c) a second reagent capable of detecting a second cell type in the body fluid.

56. The kit of claim 55, wherein the slide further comprises a channel across the surface of the slide, wherein the channel is in connection with the first chamber and the second chamber.

57. The kit of claim 55, wherein the slide further comprises a port, wherein the port is configured to receive the body fluid and pass the body fluid through the channel to at least one chamber.

58. The kit of claim 55, wherein the first cell type is a red blood cell.

59. The kit of claim 58, wherein the second cell type is a white blood cell.

60. The kit of claim 59, wherein the slide further comprises a third chamber, wherein the kit comprises a third reagent capable of detecting a third cell type in the body fluid.

61. The kit of claim 60, wherein the third cell type is a platelet.