PHASE DISTRIBUTION MEASUREMENT METHOD AND PHASE DISTRIBUTION MEASUREMENT APPARATUS
First Claim
1. A phase distribution measurement method inside a biological sample, comprising:
- taking in an optical image of a biological sample formed by a microscope that converts phase distribution into image intensity distribution while changing image contrast to form a plurality of pieces of images with different image contrasts;
calculating a component corresponding to phase distribution of the biological sample and a component corresponding to other than the phase distribution of the biological sample, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution of the biological sample to form a normalized phase component image;
breaking down the phase component image into a plurality of frequency components based on a spatial frequency of the image;
performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component formed by light refracted inside the biological sample and phase distribution of a structure component formed by light diffracted in a structure inside the biological sample; and
calculating phase distribution of the biological sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component.
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Abstract
A phase distribution measurement method inside a biological sample includes taking in an optical image of the sample formed by a microscope to form a plurality of images with different image contrasts; calculating a component corresponding to phase distribution of the sample and a component corresponding to other than the phase distribution, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution to forma normalized phase component image; breaking down the phase component image into a plurality of frequency components; performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component and phase distribution of a structure component; and calculating phase distribution of the sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component.
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Citations
20 Claims
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1. A phase distribution measurement method inside a biological sample, comprising:
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taking in an optical image of a biological sample formed by a microscope that converts phase distribution into image intensity distribution while changing image contrast to form a plurality of pieces of images with different image contrasts; calculating a component corresponding to phase distribution of the biological sample and a component corresponding to other than the phase distribution of the biological sample, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution of the biological sample to form a normalized phase component image; breaking down the phase component image into a plurality of frequency components based on a spatial frequency of the image; performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component formed by light refracted inside the biological sample and phase distribution of a structure component formed by light diffracted in a structure inside the biological sample; and calculating phase distribution of the biological sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification